Key features and details
- Rabbit polyclonal to ARHGEF38
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-ARHGEF38 antibody
DescriptionRabbit polyclonal to ARHGEF38
Tested applicationsSuitable for: ICC/IF, IHC-P, WBmore details
Species reactivityReacts with: Human
- Human skin tissue; Transfected HEK293T cell lysate.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab122345 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
|IHC-P||1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||Use a concentration of 0.04 - 0.4 µg/ml.|
FunctionMay act as a guanine-nucleotide releasing factor.
Sequence similaritiesContains 1 DH (DBL-homology) domain.
- Information by UniProt
- ARH38_HUMAN antibody
- Arhgef38 antibody
- FLJ20184 antibody
- Rho guanine nucleotide exchange factor 38 antibody
Immunofluorescent staining of Human cell line U-251 MG shows positivity in cytoskeleton (intermediate filaments). Recommended concentration of ab122345 1-4 µg/ml. Cells treated with PFA/Triton X-100.
ab122345, at a 1/150 dilution, staining ARHGEF38 in paraffin-embedded Human skin tissue by immunohistochemistry.
All lanes : Anti-ARHGEF38 antibody (ab122345) at 1/250 dilution
Lane 1 : Vector only transfected HEK293T lysate
Lane 2 : ARHGEF38-transfected HEK293T over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa))
Developed using the ECL technique.
ab122345 has not yet been referenced specifically in any publications.