Key features and details
- Goat polyclonal to ARHGEF5
- Suitable for: WB, ELISA
- Reacts with: Human
- Isotype: IgG
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituent: 0.5% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Our Abpromise guarantee covers the use of ab42695 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use at a concentration of 0.1 - 0.3 µg/ml. Predicted molecular weight: 177 kDa. Approx 170kDa band observed in Human HeLa lysates
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Tissue specificityMainly expressed in kidney, liver, pancreas, lung and placenta.
Sequence similaritiesContains 1 DH (DBL-homology) domain.
Contains 1 PH domain.
Contains 1 SH3 domain.
- Information by UniProt
- ARHG5_HUMAN antibody
- ARHGEF 5 antibody
- ARHGEF5 antibody
Anti-ARHGEF5 antibody (ab42695) at 0.1 µg/ml + HeLa Lysate (35µg protein in RIPA buffer)
Predicted band size: 177 kDa
Observed band size: 170 kDa why is the actual band size different from the predicted?
Primary incubation was 1 hour. Detected by chemiluminescence.
ab42695 has not yet been referenced specifically in any publications.