
Recombinant Anti-ARHGEF6 antibody [EPR11700(2)] (ab184569)
- Datasheet
- References (1)
- Protocols
Overview
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Product name
Anti-ARHGEF6 antibody [EPR11700(2)]
See all ARHGEF6 primary antibodies -
Description
Rabbit monoclonal [EPR11700(2)] to ARHGEF6 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human ARHGEF6 aa 700 to the C-terminus. The exact sequence is proprietary.
Database link: Q15052 -
Positive control
- Raji, Jurkat and HeLa whole cell lysate (ab150035); Human transitional cell carcinoma of bladder tissue.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol, 59% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR11700(2) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab184569 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | 1/10000 - 1/50000. Detects a band of approximately 87 kDa (predicted molecular weight: 87 kDa). | |
IHC-P | 1/50. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target
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Function
Acts as a RAC1 guanine nucleotide exchange factor (GEF). -
Tissue specificity
Ubiquitous. -
Involvement in disease
Defects in ARHGEF6 are the cause of mental retardation X-linked type 46 (MRX46) [MIM:300436]. Mental retardation is a mental disorder characterized by significantly sub-average general intellectual functioning associated with impairments in adaptative behavior and manifested during the developmental period. Non-syndromic mental retardation patients do not manifest other clinical signs. -
Sequence similarities
Contains 1 CH (calponin-homology) domain.
Contains 1 DH (DBL-homology) domain.
Contains 1 PH domain.
Contains 1 SH3 domain. - Information by UniProt
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Database links
- Entrez Gene: 9459 Human
- Entrez Gene: 73341 Mouse
- Entrez Gene: 363509 Rat
- Omim: 300267 Human
- SwissProt: Q15052 Human
- SwissProt: Q8K4I3 Mouse
- SwissProt: Q5XXR3 Rat
- Unigene: 522795 Human
see all -
Alternative names
- Alpha Pix antibody
- Alpha-Pix antibody
- ARHG6_HUMAN antibody
see all
Images
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All lanes : Anti-ARHGEF6 antibody [EPR11700(2)] (ab184569) at 1/10000 dilution
Lane 1 : Raji cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : HeLa cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 87 kDa
Observed band size: 87 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARHGEF6 antibody [EPR11700(2)] (ab184569)
Immunohistochemical analysis of paraffin-embedded Human transitional cell carcinoma of bladder tissue labeling ARHGEF6 with ab184569 at 1/50 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
References
This product has been referenced in:
- Konopaske GT et al. Dysbindin-1 contributes to prefrontal cortical dendritic arbor pathology in schizophrenia. Schizophr Res N/A:N/A (2018). Read more (PubMed: 29759351) »