Overview

  • Product name

    Anti-ARHGEF6 antibody [EPR11700(2)]
    See all ARHGEF6 primary antibodies
  • Description

    Rabbit monoclonal [EPR11700(2)] to ARHGEF6
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human ARHGEF6 aa 700 to the C-terminus. The exact sequence is proprietary.
    Database link: Q15052

  • Positive control

    • Raji, Jurkat and HeLa whole cell lysate (ab150035); Human transitional cell carcinoma of bladder tissue.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 0.05% BSA, 40% Glycerol, 59% PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR11700(2)
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab184569 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 87 kDa (predicted molecular weight: 87 kDa).
IHC-P 1/50. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

  • Function

    Acts as a RAC1 guanine nucleotide exchange factor (GEF).
  • Tissue specificity

    Ubiquitous.
  • Involvement in disease

    Defects in ARHGEF6 are the cause of mental retardation X-linked type 46 (MRX46) [MIM:300436]. Mental retardation is a mental disorder characterized by significantly sub-average general intellectual functioning associated with impairments in adaptative behavior and manifested during the developmental period. Non-syndromic mental retardation patients do not manifest other clinical signs.
  • Sequence similarities

    Contains 1 CH (calponin-homology) domain.
    Contains 1 DH (DBL-homology) domain.
    Contains 1 PH domain.
    Contains 1 SH3 domain.
  • Information by UniProt
  • Database links

  • Alternative names

    • Alpha Pix antibody
    • Alpha-Pix antibody
    • ARHG6_HUMAN antibody
    • ARHGEF6 antibody
    • COOL 2 antibody
    • COOL-2 antibody
    • COOL2 antibody
    • Mental retardation, X linked 46 antibody
    • MRX46 antibody
    • PAK interacting exchange factor alpha antibody
    • PAK-interacting exchange factor alpha antibody
    • PIXA antibody
    • Rac/Cdc42 guanine exchange factor (GEF) 6 antibody
    • Rac/Cdc42 guanine nucleotide exchange factor (GEF) 6 antibody
    • Rac/Cdc42 guanine nucleotide exchange factor 6 antibody
    • Rho guanine nucleotide exchange factor 6 antibody
    see all

Images

  • All lanes : Anti-ARHGEF6 antibody [EPR11700(2)] (ab184569) at 1/10000 dilution

    Lane 1 : Raji cell lysate
    Lane 2 : Jurkat cell lysate
    Lane 3 : HeLa cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 87 kDa
    Observed band size: 87 kDa

  • Immunohistochemical analysis of paraffin-embedded Human transitional cell carcinoma of bladder tissue labeling ARHGEF6 with ab184569 at 1/50 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

     

     

     

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

References

This product has been referenced in:

  • Konopaske GT  et al. Dysbindin-1 contributes to prefrontal cortical dendritic arbor pathology in schizophrenia. Schizophr Res N/A:N/A (2018). Read more (PubMed: 29759351) »
See 1 Publication for this product

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