RecombinantRabMAb

Recombinant Anti-ARHGEF6 antibody [EPR11700(2)] (ab184569)

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Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR11700(2)] to ARHGEF6
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-ARHGEF6 antibody [EPR11700(2)]
    See all ARHGEF6 primary antibodies

  • Description

    Rabbit monoclonal [EPR11700(2)] to ARHGEF6

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human ARHGEF6 aa 700 to the C-terminus. The exact sequence is proprietary.
    Database link: Q15052

  • Positive control

    • Raji, Jurkat and HeLa whole cell lysate (ab150035); Human transitional cell carcinoma of bladder tissue.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 0.05% BSA, 40% Glycerol, 59% PBS
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR11700(2)

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab184569 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 87 kDa (predicted molecular weight: 87 kDa).
IHC-P 1/50. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

  • Function

    Acts as a RAC1 guanine nucleotide exchange factor (GEF).

  • Tissue specificity

    Ubiquitous.

  • Involvement in disease

    Defects in ARHGEF6 are the cause of mental retardation X-linked type 46 (MRX46) [MIM:300436]. Mental retardation is a mental disorder characterized by significantly sub-average general intellectual functioning associated with impairments in adaptative behavior and manifested during the developmental period. Non-syndromic mental retardation patients do not manifest other clinical signs.

  • Sequence similarities

    Contains 1 CH (calponin-homology) domain.
    Contains 1 DH (DBL-homology) domain.
    Contains 1 PH domain.
    Contains 1 SH3 domain.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • Alpha Pix antibody
    • Alpha-Pix antibody
    • ARHG6_HUMAN antibody
    • ARHGEF6 antibody
    • COOL 2 antibody
    • COOL-2 antibody
    • COOL2 antibody
    • Mental retardation, X linked 46 antibody
    • MRX46 antibody
    • PAK interacting exchange factor alpha antibody
    • PAK-interacting exchange factor alpha antibody
    • PIXA antibody
    • Rac/Cdc42 guanine exchange factor (GEF) 6 antibody
    • Rac/Cdc42 guanine nucleotide exchange factor (GEF) 6 antibody
    • Rac/Cdc42 guanine nucleotide exchange factor 6 antibody
    • Rho guanine nucleotide exchange factor 6 antibody
    see all

Images

  • Western blot - Anti-ARHGEF6 antibody [EPR11700(2)] (ab184569)
    Western blot - Anti-ARHGEF6 antibody [EPR11700(2)] (ab184569)
    All lanes : Anti-ARHGEF6 antibody [EPR11700(2)] (ab184569) at 1/10000 dilution

    Lane 1 : Raji cell lysate
    Lane 2 : Jurkat cell lysate
    Lane 3 : HeLa cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 87 kDa
    Observed band size: 87 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARHGEF6 antibody [EPR11700(2)] (ab184569)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARHGEF6 antibody [EPR11700(2)] (ab184569)

    Immunohistochemical analysis of paraffin-embedded Human transitional cell carcinoma of bladder tissue labeling ARHGEF6 with ab184569 at 1/50 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

     

     

     

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Anti-ARHGEF6 antibody [EPR11700(2)] (ab184569)
    Anti-ARHGEF6 antibody [EPR11700(2)] (ab184569)

References (1)

Publishing research using ab184569? Please let us know so that we can cite the reference in this datasheet.

ab184569 has been referenced in 1 publication.

  • Konopaske GT  et al. Dysbindin-1 contributes to prefrontal cortical dendritic arbor pathology in schizophrenia. Schizophr Res N/A:N/A (2018). PubMed: 29759351

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