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Signal Transduction Signaling Pathway G Protein Signaling Small G Proteins Regulators
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RecombinantRabMAb

Recombinant Anti-ARHGEF6 antibody [EPR11700(2)] - BSA and Azide free (ab250770)

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  • Certificate of Compliance
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Western blot - Anti-ARHGEF6 antibody [EPR11700(2)] - BSA and Azide free (ab250770)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARHGEF6 antibody [EPR11700(2)] - BSA and Azide free (ab250770)
  • Anti-ARHGEF6 antibody [EPR11700(2)] - BSA and Azide free (ab250770)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR11700(2)] to ARHGEF6 - BSA and Azide free
  • Suitable for: IHC-P, WB
  • Reacts with: Mouse, Rat, Human

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Recombinant Human ARHGEF6 protein (ab160497)
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Overview

  • Product name

    Anti-ARHGEF6 antibody [EPR11700(2)] - BSA and Azide free
    See all ARHGEF6 primary antibodies
  • Description

    Rabbit monoclonal [EPR11700(2)] to ARHGEF6 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human ARHGEF6 aa 700 to the C-terminus. The exact sequence is proprietary.
    Database link: Q15052

  • General notes

    Ab250770 is the carrier-free version of ab184569. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab250770 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR11700(2)
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Signaling Pathway
    • G Protein Signaling
    • Small G Proteins
    • Regulators

Associated products

  • Alternative Versions

    • Anti-ARHGEF6 antibody [EPR11700(2)] (ab184569)
  • Conjugation kits

    • FITC Conjugation Kit (Fast) - Lightning-Link® (ab188285)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control - BSA and Azide Free (ab210849)
  • Positive Controls

    • HeLa whole cell lysate (ab150035)
    • HeLa whole cell lysate (ab29545)
    • Raji whole cell lysate (ab30124)
    • Jurkat whole cell lysate (ab30128)
    • Jurkat whole cell lysate (ab7899)
  • Recombinant Protein

    • Recombinant Human ARHGEF6 protein (ab160497)
  • Related Products

    • Recombinant Human ARHGEF6 protein (ab160497)

Applications

Our Abpromise guarantee covers the use of ab250770 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB Use at an assay dependent concentration. Detects a band of approximately 87 kDa (predicted molecular weight: 87 kDa).

Target

  • Function

    Acts as a RAC1 guanine nucleotide exchange factor (GEF).
  • Tissue specificity

    Ubiquitous.
  • Involvement in disease

    Defects in ARHGEF6 are the cause of mental retardation X-linked type 46 (MRX46) [MIM:300436]. Mental retardation is a mental disorder characterized by significantly sub-average general intellectual functioning associated with impairments in adaptative behavior and manifested during the developmental period. Non-syndromic mental retardation patients do not manifest other clinical signs.
  • Sequence similarities

    Contains 1 CH (calponin-homology) domain.
    Contains 1 DH (DBL-homology) domain.
    Contains 1 PH domain.
    Contains 1 SH3 domain.
  • Target information above from: UniProt accession Q15052 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 9459 Human
    • Entrez Gene: 73341 Mouse
    • Entrez Gene: 363509 Rat
    • Omim: 300267 Human
    • SwissProt: Q15052 Human
    • SwissProt: Q8K4I3 Mouse
    • SwissProt: Q5XXR3 Rat
    • Unigene: 522795 Human
    • Unigene: 261443 Mouse
    • Unigene: 208975 Rat
    see all
  • Alternative names

    • Alpha Pix antibody
    • Alpha-Pix antibody
    • ARHG6_HUMAN antibody
    • ARHGEF6 antibody
    • COOL 2 antibody
    • COOL-2 antibody
    • COOL2 antibody
    • Mental retardation, X linked 46 antibody
    • MRX46 antibody
    • PAK interacting exchange factor alpha antibody
    • PAK-interacting exchange factor alpha antibody
    • PIXA antibody
    • Rac/Cdc42 guanine exchange factor (GEF) 6 antibody
    • Rac/Cdc42 guanine nucleotide exchange factor (GEF) 6 antibody
    • Rac/Cdc42 guanine nucleotide exchange factor 6 antibody
    • Rho guanine nucleotide exchange factor 6 antibody
    see all

Images

  • Western blot - Anti-ARHGEF6 antibody [EPR11700(2)] - BSA and Azide free (ab250770)
    Western blot - Anti-ARHGEF6 antibody [EPR11700(2)] - BSA and Azide free (ab250770)
    All lanes : Anti-ARHGEF6 antibody [EPR11700(2)] (ab184569) at 1/10000 dilution

    Lane 1 : Raji cell lysate
    Lane 2 : Jurkat cell lysate
    Lane 3 : HeLa cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 87 kDa
    Observed band size: 87 kDa



    This data was developed using ab184569, the same antibody clone in a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARHGEF6 antibody [EPR11700(2)] - BSA and Azide free (ab250770)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARHGEF6 antibody [EPR11700(2)] - BSA and Azide free (ab250770)
    This data was developed using ab184569, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human transitional cell carcinoma of bladder tissue labeling ARHGEF6 with ab184569 at 1/50 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin. Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
  • Anti-ARHGEF6 antibody [EPR11700(2)] - BSA and Azide free (ab250770)
    Anti-ARHGEF6 antibody [EPR11700(2)] - BSA and Azide free (ab250770)

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

    • Datasheet

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

  • References (0)

    Publishing research using ab250770? Please let us know so that we can cite the reference in this datasheet.

    ab250770 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    There are currently no Customer reviews or Questions for ab250770.
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