Product nameAnti-ARHGEF7 antibody
See all ARHGEF7 primary antibodies
DescriptionRabbit polyclonal to ARHGEF7
Tested applicationsSuitable for: ICC/IF, IPmore details
Species reactivityReacts with: Human
Recombinant fragment corresponding to Human ARHGEF7 aa 1-250.
Database link: Q14155
- ICC/IF: HeLa cells. IP: HeLa whole cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.03% Proclin
Constituents: 50% Glycerol, PBS
Concentration information loading...
PurityProtein G purified
Purification notesPurity >95%.
Our Abpromise guarantee covers the use of ab236103 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/200 - 1/500.|
|IP||1/200 - 1/2000.|
FunctionActs as a RAC1 guanine nucleotide exchange factor (GEF) and can induce membrane ruffling. May function as a positive regulator of apoptosis. May function in cell migration.
Sequence similaritiesContains 1 CH (calponin-homology) domain.
Contains 1 DH (DBL-homology) domain.
Contains 1 PH domain.
Contains 1 SH3 domain.
- Information by UniProt
- ARHG7 antibody
- ARHG7_HUMAN antibody
- ARHGEF 7 antibody
HeLa (Human epithelial cell line from cervix adenocarcinoma) cells stained for ARHGEF7 (Green) using ab236103 at 1/250 dilution in ICC/IF, followed by Alexa Fluor 488® conjugated Goat Anti-Rabbit IgG (H+L). Counterstained with DAPI (Blue).
The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal goat serum. The cells were then incubated with the primary antibody overnight at 4°C.
ARHGEF7 was immunoprecipitated from HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate using ab236103 at 1/200 dilution.
Lane 1: Rabbit control IgG (1 µg) in HeLa whole cell lysate.
Lane 2: ab236103 in HeLa whole cell lysate.
Lane 3: HeLa whole cell lysate (10 µg input).
For western blotting an HRP-conjugated Protein G antibody was used as the secondary antibody at 1/2000 dilution.
ab236103 has not yet been referenced specifically in any publications.