Key features and details
- Rabbit polyclonal to ARHGEF7
- Suitable for: ICC/IF, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-ARHGEF7 antibody
See all ARHGEF7 primary antibodies
DescriptionRabbit polyclonal to ARHGEF7
Tested applicationsSuitable for: ICC/IF, IPmore details
Species reactivityReacts with: Human
Recombinant fragment corresponding to Human ARHGEF7 aa 1-250.
Database link: Q14155
- ICC/IF: HeLa cells. IP: HeLa whole cell lysate.
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We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Purification notesPurity >95%.
Our Abpromise guarantee covers the use of ab236103 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/200 - 1/500.|
|IP||1/200 - 1/2000.|
FunctionActs as a RAC1 guanine nucleotide exchange factor (GEF) and can induce membrane ruffling. May function as a positive regulator of apoptosis. May function in cell migration.
Sequence similaritiesContains 1 CH (calponin-homology) domain.
Contains 1 DH (DBL-homology) domain.
Contains 1 PH domain.
Contains 1 SH3 domain.
- Information by UniProt
- ARHG7 antibody
- ARHG7_HUMAN antibody
- ARHGEF 7 antibody
HeLa (Human epithelial cell line from cervix adenocarcinoma) cells stained for ARHGEF7 (Green) using ab236103 at 1/250 dilution in ICC/IF, followed by Alexa Fluor 488® conjugated Goat Anti-Rabbit IgG (H+L). Counterstained with DAPI (Blue).
The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal goat serum. The cells were then incubated with the primary antibody overnight at 4°C.
ARHGEF7 was immunoprecipitated from HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate using ab236103 at 1/200 dilution.
Lane 1: Rabbit control IgG (1 µg) in HeLa whole cell lysate.
Lane 2: ab236103 in HeLa whole cell lysate.
Lane 3: HeLa whole cell lysate (10 µg input).
For western blotting an HRP-conjugated Protein G antibody was used as the secondary antibody at 1/2000 dilution.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab236103 has not yet been referenced specifically in any publications.