Overview

  • Product name

  • Description

    Rabbit polyclonal to ARHGEF7
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, IPmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human ARHGEF7 aa 1-250.
    Database link: Q14155

  • Positive control

    • ICC/IF: HeLa cells. IP: HeLa whole cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab236103 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/200 - 1/500.
IP 1/200 - 1/2000.

Target

  • Function

    Acts as a RAC1 guanine nucleotide exchange factor (GEF) and can induce membrane ruffling. May function as a positive regulator of apoptosis. May function in cell migration.
  • Sequence similarities

    Contains 1 CH (calponin-homology) domain.
    Contains 1 DH (DBL-homology) domain.
    Contains 1 PH domain.
    Contains 1 SH3 domain.
  • Information by UniProt
  • Database links

  • Alternative names

    • ARHG7 antibody
    • ARHG7_HUMAN antibody
    • ARHGEF 7 antibody
    • Arhgef7 antibody
    • Beta pix antibody
    • Beta-Pix antibody
    • betaPix antibody
    • betaPix-b antibody
    • betaPix-c antibody
    • Cool antibody
    • COOL-1 antibody
    • COOL1 antibody
    • DKFZp686C12170 antibody
    • DKFZp761K1021 antibody
    • KIAA0142 antibody
    • KIAA0412 antibody
    • mKIAA0142 antibody
    • Nbla10314 antibody
    • P50 antibody
    • P50BP antibody
    • p85 antibody
    • P85COOL1 antibody
    • P85SPR antibody
    • PAK interacting exchange factor beta antibody
    • PAK-interacting exchange factor beta antibody
    • PAK3 antibody
    • Pak3bp antibody
    • PIX antibody
    • PIXB antibody
    • Rho guanine nucleotide exchange factor (GEF) 7 antibody
    • Rho guanine nucleotide exchange factor (GEF) 7b antibody
    • Rho guanine nucleotide exchange factor (GEF7) antibody
    • Rho guanine nucleotide exchange factor 7 antibody
    • SH3 domain containing proline rich protein antibody
    see all

Images

  • HeLa (Human epithelial cell line from cervix adenocarcinoma) cells stained for ARHGEF7 (Green) using ab236103 at 1/250 dilution in ICC/IF, followed by Alexa Fluor 488® conjugated Goat Anti-Rabbit IgG (H+L). Counterstained with DAPI (Blue).

    The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal goat serum. The cells were then incubated with the primary antibody overnight at 4°C.

  • ARHGEF7 was immunoprecipitated from HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate using ab236103 at 1/200 dilution.

    Lane 1: Rabbit control IgG (1 µg) in HeLa whole cell lysate.

    Lane 2: ab236103 in HeLa whole cell lysate.

    Lane 3: HeLa whole cell lysate (10 µg input).

    For western blotting an HRP-conjugated Protein G antibody was used as the secondary antibody at 1/2000 dilution.

References

ab236103 has not yet been referenced specifically in any publications.

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