Key features and details
- Rabbit polyclonal to ARHI
- Suitable for: WB, Flow Cyt, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-ARHI antibody
See all ARHI primary antibodies
DescriptionRabbit polyclonal to ARHI
Tested applicationsSuitable for: WB, Flow Cyt, IHC-Pmore details
Species reactivityReacts with: Human
Recombinant protein of Human ARHI (NP_004666.1).
- WB: WiDr cell line lysates IHC-P: Human brain tissue
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C long term.
Storage bufferPreservative: 0.09% Sodium azide
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab107051 is purified through a protein A column, followed by peptide affinity purification.
Our Abpromise guarantee covers the use of ab107051 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/100 - 1/500. Predicted molecular weight: 26 kDa.|
|Flow Cyt||1/10 - 1/50.
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
|IHC-P||1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
Tissue specificityExpressed in normal ovarian and breast epithelial cells but not in ovarian and breast cancers.
Sequence similaritiesBelongs to the small GTPase superfamily. Di-Ras family.
Cellular localizationCell membrane.
- Information by UniProt
- DIRA3 antibody
- DIRA3_HUMAN antibody
- DIRAS family GTP binding RAS like 3 antibody
Anti-ARHI antibody (ab107051) at 1/100 dilution + WiDr cell line lysates at 35 µg
Predicted band size: 26 kDa
ab107051 flow cytometric analysis of WiDr cells (right histogram) compared to a hegative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
ab107051, at a1/50 dilution, staining ARHI in formalin fixed and paraffin embedded Human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
ab107051 has been referenced in 3 publications.
- Zhong C et al. Oncogenic Ras is downregulated by ARHI and induces autophagy by Ras/AKT/mTOR pathway in glioblastoma. BMC Cancer 19:441 (2019). PubMed: 31088402
- Ouyang J et al. The role of aplysia ras homolog I in colon cancer cell invasion and adhesion. Exp Ther Med 14:5193-5199 (2017). PubMed: 29201236
- Li LL et al. JMJD2A contributes to breast cancer progression through transcriptional repression of the tumor suppressor ARHI. Breast Cancer Res 16:R56 (2014). PubMed: 24886710