Overview

  • Product name
    Anti-ARID1A antibody - C-terminal
    See all ARID1A primary antibodies
  • Description
    Rabbit polyclonal to ARID1A - C-terminal
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Rat, Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide corresponding to Human ARID1A aa 2199-2213 (C terminal).
    Sequence:

    LEDSLAATQFQQSQA


    Database link: O14497

  • Positive control
    • HeLa and K562 cells; SKOV, HeLa, Jurkat, U937, COLO320 and rat thymus lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab176395 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.1 - 0.5 µg/ml. Predicted molecular weight: 242 kDa.

Target

  • Function
    Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Binds DNA non-specifically. Also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene. Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth.
  • Tissue specificity
    Highly expressed in spleen, thymus, prostate, testis, ovary, small intestine, colon, and PBL, and at a much lower level in heart, brain, placenta, lung, liver, skeletal muscle, kidney, and pancreas.
  • Sequence similarities
    Contains 1 ARID domain.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • actin-dependent regulator of chromatin subfamily F member 1 antibody
    • ARI1A_HUMAN antibody
    • ARID domain containing protein 1A antibody
    • ARID domain-containing protein 1A antibody
    • ARID1A antibody
    • AT rich interactive domain 1A (SWI like) antibody
    • AT rich interactive domain 1A antibody
    • AT rich interactive domain containing protein 1A antibody
    • AT-rich interactive domain-containing protein 1A antibody
    • B120 antibody
    • BAF250 antibody
    • BAF250A antibody
    • BM029 antibody
    • brain protein 120 antibody
    • BRG1 associated factor 250 antibody
    • BRG1 associated factor 250a antibody
    • BRG1-associated factor 250 antibody
    • BRG1-associated factor 250a antibody
    • C1ORF4 antibody
    • chromatin remodeling factor p250 antibody
    • chromosome 1 open reading frame 4 antibody
    • ELD antibody
    • hELD antibody
    • hOSA1 antibody
    • matrix-associated antibody
    • MRD14 antibody
    • Osa homolog 1 antibody
    • OSA1 antibody
    • OSA1 nuclear protein antibody
    • P270 antibody
    • SMARCF1 antibody
    • SWI like protein antibody
    • SWI SNF complex protein p270 antibody
    • SWI-like protein antibody
    • SWI/SNF complex protein p270 antibody
    • SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily f, member 1 antibody
    • SWI/SNF-related antibody
    see all

Images

  • All lanes : Anti-ARID1A antibody - C-terminal (ab176395) at 0.5 µg/ml

    Lane 1 : SKOV cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : Jurkat cell lysate
    Lane 4 : U937 cell lysate

    Predicted band size: 242 kDa

  • All lanes : Anti-ARID1A antibody - C-terminal (ab176395) at 0.5 µg/ml

    Lane 1 : Rat thymus lysate
    Lane 2 : COLO320 cell lysate
    Lane 3 : SKOV cell lysate

    Predicted band size: 242 kDa

References

This product has been referenced in:
  • Zhang L  et al. Loss of ARID1A Expression Correlates With Tumor Differentiation and Tumor Progression Stage in Pancreatic Ductal Adenocarcinoma. Technol Cancer Res Treat 17:1533034618754475 (2018). Read more (PubMed: 29486633) »
  • Davidson J  et al. SWI/SNF aberrations sensitize pancreatic cancer cells to DNA crosslinking agents. Oncotarget 9:9608-9617 (2018). Read more (PubMed: 29515757) »
See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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