Overview

  • Product name

    Anti-ARID1A antibody - C-terminal
    See all ARID1A primary antibodies
  • Description

    Rabbit polyclonal to ARID1A - C-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human ARID1A (C terminal). The exact sequence is proprietary.
    Database link: O14497

  • Positive control

    • WB: Human ARID1A-transfected HEK-293T whole cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab227862 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 242 kDa.

Target

  • Function

    Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Binds DNA non-specifically. Also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene. Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth.
  • Tissue specificity

    Highly expressed in spleen, thymus, prostate, testis, ovary, small intestine, colon, and PBL, and at a much lower level in heart, brain, placenta, lung, liver, skeletal muscle, kidney, and pancreas.
  • Sequence similarities

    Contains 1 ARID domain.
  • Post-translational
    modifications

    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • actin-dependent regulator of chromatin subfamily F member 1 antibody
    • ARI1A_HUMAN antibody
    • ARID domain containing protein 1A antibody
    • ARID domain-containing protein 1A antibody
    • ARID1A antibody
    • AT rich interactive domain 1A (SWI like) antibody
    • AT rich interactive domain 1A antibody
    • AT rich interactive domain containing protein 1A antibody
    • AT-rich interactive domain-containing protein 1A antibody
    • B120 antibody
    • BAF250 antibody
    • BAF250A antibody
    • BM029 antibody
    • brain protein 120 antibody
    • BRG1 associated factor 250 antibody
    • BRG1 associated factor 250a antibody
    • BRG1-associated factor 250 antibody
    • BRG1-associated factor 250a antibody
    • C1ORF4 antibody
    • chromatin remodeling factor p250 antibody
    • chromosome 1 open reading frame 4 antibody
    • ELD antibody
    • hELD antibody
    • hOSA1 antibody
    • matrix-associated antibody
    • MRD14 antibody
    • Osa homolog 1 antibody
    • OSA1 antibody
    • OSA1 nuclear protein antibody
    • P270 antibody
    • SMARCF1 antibody
    • SWI like protein antibody
    • SWI SNF complex protein p270 antibody
    • SWI-like protein antibody
    • SWI/SNF complex protein p270 antibody
    • SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily f, member 1 antibody
    • SWI/SNF-related antibody
    see all

Images

  • All lanes : Anti-ARID1A antibody - C-terminal (ab227862) at 1/5000 dilution

    Lane 1 : Non-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
    Lane 2 : Human ARID1A-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 242 kDa



    5% SDS-PAGE gel.

References

ab227862 has not yet been referenced specifically in any publications.

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