Anti-ARID1B antibody [2D2] (ab57461)
Key features and details
- Mouse monoclonal [2D2] to ARID1B
- Suitable for: WB, IHC-P, ICC/IF, Flow Cyt
- Reacts with: Human
- Isotype: IgG2b
Overview
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Product name
Anti-ARID1B antibody [2D2]
See all ARID1B primary antibodies -
Description
Mouse monoclonal [2D2] to ARID1B -
Host species
Mouse -
Tested applications
Suitable for: WB, IHC-P, ICC/IF, Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment: PPAKRHEGDM YNMQYSSQQQ EMYNQYGGSY SGPDRRPIQG QYPYPYSRER MQGPGQIQTH GIPPQMMGGP LQSSSSEGPQ QNMWAARNDM PYPYQNR, corresponding to amino acids 1364-1461 of Human ARID1B
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Positive control
- ICC/IF: HepG2 cells.
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General notes
This product was changed from ascites to tissue culture supernatant on 12 Feb 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.40
Constituent: 100% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
2D2 -
Isotype
IgG2b -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab57461 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
Use at an assay dependent concentration. Predicted molecular weight: 236 kDa.
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IHC-P | (1) |
Use at an assay dependent concentration.
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ICC/IF |
Use at an assay dependent concentration.
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Flow Cyt |
Use at an assay dependent concentration.
ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
Notes |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 236 kDa. |
IHC-P
Use at an assay dependent concentration. |
ICC/IF
Use at an assay dependent concentration. |
Flow Cyt
Use at an assay dependent concentration. ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
Target
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Relevance
ARID1B (AT-rich interactive domain-containing protein 1B) is a component of SWI/SNF chromatin remodeling complexes involved in transcriptional activation and repression of select genes by chromatin remodeling. It binds DNA non-specifically and alters DNA-nucleosome topology. -
Cellular localization
Nuclear -
Database links
- Entrez Gene: 57492 Human
- Omim: 614556 Human
- SwissProt: Q8NFD5 Human
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Alternative names
- 6A3 5 antibody
- ARID 1B antibody
- ARID domain containing protein 1B antibody
see all
Images
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Anti-ARID1B antibody [2D2] (ab57461) at 5 µg/ml + HeLa (human cervix adenocarcinoma epithelial cell) Nuclear Extract at 50 µg
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution
Predicted band size: 236 kDaBlocking and diluting buffer and concentration: 5% non-fat milk/PBST (0.2%).
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Immunoperoxidase analysis of formalin-fixed paraffin-embedded human kidney tissue labelling ARID1B with ab129165 at 3 µg/mL concentration.
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Immunocytochemistry/Immunofluorescence analysis of HeLa (human cervix adenocarcinoma epithelial cell) cells labelling ARID1B (Green) with ab129165 at 10 µg/mL concentration.
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Sandwich ELSIA standard curve using ab57461 as a capture antibody. Detection limit for recombinant GST tagged ARID1B is 0.03 ng/mL.
Protocols
Datasheets and documents
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Datasheet download
References (25)
ab57461 has been referenced in 25 publications.
- Kim H et al. Early postnatal serotonin modulation prevents adult-stage deficits in Arid1b-deficient mice through synaptic transcriptional reprogramming. Nat Commun 13:5051 (2022). PubMed: 36030255
- Moffat JJ et al. Differential roles of ARID1B in excitatory and inhibitory neural progenitors in the developing cortex. Sci Rep 11:3856 (2021). PubMed: 33594090
- Saqcena M et al. SWI/SNF Complex Mutations Promote Thyroid Tumor Progression and Insensitivity to Redifferentiation Therapies. Cancer Discov 11:1158-1175 (2021). PubMed: 33318036
- Yang QQ et al. Nuclear isoform of FGF13 regulates post-natal neurogenesis in the hippocampus through an epigenomic mechanism. Cell Rep 35:109127 (2021). PubMed: 34010636
- Li J et al. A Role for SMARCB1 in Synovial Sarcomagenesis Reveals That SS18-SSX Induces Canonical BAF Destruction. Cancer Discov 11:2620-2637 (2021). PubMed: 34078620