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    arid2-antibody-ab113283.pdf

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Epigenetics and Nuclear Signaling Chromatin Remodeling Swi / Snf
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Anti-ARID2 antibody (ab113283)

  • Datasheet
  • SDS
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARID2 antibody (ab113283)

    Key features and details

    • Rabbit polyclonal to ARID2
    • Suitable for: IHC-P
    • Reacts with: Human
    • Isotype: IgG

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    Overview

    • Product name

      Anti-ARID2 antibody
      See all ARID2 primary antibodies
    • Description

      Rabbit polyclonal to ARID2
    • Host species

      Rabbit
    • Tested applications

      Suitable for: IHC-Pmore details
    • Species reactivity

      Reacts with: Human
      Predicted to work with: Horse, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Opossum, Orangutan, Bat
    • Immunogen

      Synthetic peptide corresponding to a region within amino acids 1785-1835 of Human ARID2 (NP_689854.2).

    • General notes

      Concentration is optimized for IHC and not determined

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
    • Storage buffer

      pH: 6.8
      Preservative: 0.09% Sodium azide
      Constituents: 99% Tris buffered saline, 0.1% BSA
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Epigenetics and Nuclear Signaling
      • Chromatin Remodeling
      • Swi / Snf
      • Epigenetics and Nuclear Signaling
      • Transcription
      • Other factors

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab113283 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    IHC-P
    1/100 - 1/500. Epitope exposure is recommended. Epitope exposure with citrate buffer will enhance staining.
    Notes
    IHC-P
    1/100 - 1/500. Epitope exposure is recommended. Epitope exposure with citrate buffer will enhance staining.

    Target

    • Function

      Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Required for the stability of the SWI/SNF chromatin remodeling complex SWI/SNF-B (PBAF). May be involved in targeting the complex to different genes. May be involved in regulating transcriptional activation of cardiac genes.
    • Tissue specificity

      Highly expressed in heart and testis.
    • Sequence similarities

      Contains 1 ARID domain.
      Contains 1 C2H2-type zinc finger.
      Contains 1 RFX-type winged-helix DNA-binding domain.
    • Developmental stage

      Up-regulated in adult heart (at protein level).
    • Cellular localization

      Nucleus.
    • Target information above from: UniProt accession Q68CP9 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 196528 Human
      • Omim: 609539 Human
      • SwissProt: Q68CP9 Human
      • Unigene: 317304 Human
      • Alternative names

        • ARID domain containing protein 2 antibody
        • ARID domain-containing protein 2 antibody
        • ARID2 antibody
        • ARID2_HUMAN antibody
        • AT rich interactive domain 2 (ARID, RFX like) antibody
        • AT rich interactive domain containing protein 2 antibody
        • AT-rich interactive domain-containing protein 2 antibody
        • BAF antibody
        • BAF200 antibody
        • BRG1 associated factor 200 antibody
        • BRG1-associated factor 200 antibody
        • DKFZp686G052 antibody
        • DKFZp779P0222 antibody
        • FLJ30619 antibody
        • KIAA1557 antibody
        • p200 antibody
        • Zinc finger protein with activation potential antibody
        • zip zap antibody
        • zipzap antibody
        • Zipzap/p200 antibody
        see all

      Images

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARID2 antibody (ab113283)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARID2 antibody (ab113283)
        Staining of Human ARID2 in a Formalin/PFA-fixed paraffin-embedded section of Human Testicular Seminoma using ab113283 at a dilution of 1/250.

      Protocols

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      Datasheets and documents

      • SDS download

      • Datasheet download

        Download

      References (2)

      Publishing research using ab113283? Please let us know so that we can cite the reference in this datasheet.

      ab113283 has been referenced in 2 publications.

      • Cabot B  et al. Differential expression of key subunits of SWI/SNF chromatin remodeling complexes in porcine embryos derived in vitro or in vivo. Mol Reprod Dev 84:1238-1249 (2017). ICC/IF . PubMed: 29024220
      • Gao Q  et al. HBx protein-mediated ATOH1 downregulation suppresses ARID2 expression and promotes hepatocellular carcinoma. Cancer Sci 108:1328-1337 (2017). WB, IHC-P . PubMed: 28498550

      Customer reviews and Q&As

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