Anti-ARID2 antibody (ab113283)
- Datasheet
- References (2)
- Protocols
Overview
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Product name
Anti-ARID2 antibody
See all ARID2 primary antibodies -
Description
Rabbit polyclonal to ARID2 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Horse, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Opossum, Orangutan, Bat
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Immunogen
Synthetic peptide corresponding to a region within amino acids 1785-1835 of Human ARID2 (NP_689854.2).
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Positive control
- Human Breast Carcinoma, Human Prostate Carcinoma, and Human Testicular Seminoma tissues This antibody gave a positive result when used in the following methanol fixed cell lines: MCF-7.
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General notes
Concentration is optimized for IHC and not determined
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 99% Tris buffered saline, 0.1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab113283 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | 1/100 - 1/500. Epitope exposure is recommended. Epitope exposure with citrate buffer will enhance staining. | |
| ICC/IF | 1/200. |
Target
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Function
Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Required for the stability of the SWI/SNF chromatin remodeling complex SWI/SNF-B (PBAF). May be involved in targeting the complex to different genes. May be involved in regulating transcriptional activation of cardiac genes. -
Tissue specificity
Highly expressed in heart and testis. -
Sequence similarities
Contains 1 ARID domain.
Contains 1 C2H2-type zinc finger.
Contains 1 RFX-type winged-helix DNA-binding domain. -
Developmental stage
Up-regulated in adult heart (at protein level). -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 196528 Human
- Omim: 609539 Human
- SwissProt: Q68CP9 Human
- Unigene: 317304 Human
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Alternative names
- ARID domain containing protein 2 antibody
- ARID domain-containing protein 2 antibody
- ARID2 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARID2 antibody (ab113283)Staining of Human ARID2 in a Formalin/PFA-fixed paraffin-embedded section of Human Testicular Seminoma using ab113283 at a dilution of 1/250. -
Immunocytochemistry/ Immunofluorescence - Anti-ARID2 antibody (ab113283)ab113283 stained MCF-7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab113283 at 1/200 dilution overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Datasheets and documents
References
This product has been referenced in:
- Cabot B et al. Differential expression of key subunits of SWI/SNF chromatin remodeling complexes in porcine embryos derived in vitro or in vivo. Mol Reprod Dev 84:1238-1249 (2017). ICC/IF . Read more (PubMed: 29024220) »
- Gao Q et al. HBx protein-mediated ATOH1 downregulation suppresses ARID2 expression and promotes hepatocellular carcinoma. Cancer Sci 108:1328-1337 (2017). WB, IHC-P . Read more (PubMed: 28498550) »
