Product nameAnti-ARID2 antibody
See all ARID2 primary antibodies
DescriptionRabbit polyclonal to ARID2
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Sheep, Rabbit, Horse, Chicken, Cow, Pig, Chimpanzee, Macaque monkey, Gorilla, Chinese hamster, Orangutan
Synthetic peptide within Human ARID2 aa 1500-1600 conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
Database link: Q68CP9
- This antibody gave a positive signal in Human Testis tissue lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab166850 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 240 kDa (predicted molecular weight: 197 kDa).|
FunctionInvolved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Required for the stability of the SWI/SNF chromatin remodeling complex SWI/SNF-B (PBAF). May be involved in targeting the complex to different genes. May be involved in regulating transcriptional activation of cardiac genes.
Tissue specificityHighly expressed in heart and testis.
Sequence similaritiesContains 1 ARID domain.
Contains 1 C2H2-type zinc finger.
Contains 1 RFX-type winged-helix DNA-binding domain.
Developmental stageUp-regulated in adult heart (at protein level).
- Information by UniProt
- ARID domain containing protein 2 antibody
- ARID domain-containing protein 2 antibody
- ARID2 antibody
Anti-ARID2 antibody (ab166850) at 1 µg/ml (Milk blocking 3%) + Human testis tissue lysate (total protein) at 20 µg
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 197 kDa
Observed band size: 240 kDa why is the actual band size different from the predicted?
Exposure time: 20 minutes
This blot was produced using a 3-8% Tris Acetate gel under the TA buffer system. The gel was run at 150V for 60 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab166850 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.