Key features and details
- Rabbit polyclonal to ARID2
- Suitable for: IP, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-ARID2 antibody
See all ARID2 primary antibodies
DescriptionRabbit polyclonal to ARID2
Tested applicationsSuitable for: IP, WB, IHC-Pmore details
Species reactivityReacts with: Human
Synthetic peptide within Human ARID2 aa 1785-1835. The exact sequence is proprietary. NP_689854.2
Database link: Q68CP9
- WB: HeLa, HEK-293T and Jurkat whole cell lysates. IP: HeLa whole cell lysate. IHC-P: Human prostate carcinoma and ovarian carcinoma tissue.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab245530 was affinity purified using an epitope specific to ARID2 immobilized on solid support.
Our Abpromise guarantee covers the use of ab245530 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at 5-10 µg/mg of lysate.|
|WB||1/2000 - 1/10000. Predicted molecular weight: 197 kDa.|
|IHC-P||1/1000 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionInvolved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Required for the stability of the SWI/SNF chromatin remodeling complex SWI/SNF-B (PBAF). May be involved in targeting the complex to different genes. May be involved in regulating transcriptional activation of cardiac genes.
Tissue specificityHighly expressed in heart and testis.
Sequence similaritiesContains 1 ARID domain.
Contains 1 C2H2-type zinc finger.
Contains 1 RFX-type winged-helix DNA-binding domain.
Developmental stageUp-regulated in adult heart (at protein level).
- Information by UniProt
- ARID domain containing protein 2 antibody
- ARID domain-containing protein 2 antibody
- ARID2 antibody
Formalin-fixed, paraffin-embedded human prostate carcinoma tissue stained for ARID2 using ab245530 at 1/5000 dilution in immunohistochemical analysis. Detection: DAB staining.
Formalin-fixed, paraffin-embedded human ovarian carcinoma tissue stained for ARID2 using ab245530 at 1/5000 dilution in immunohistochemical analysis. Detection: DAB staining.
All lanes : Anti-ARID2 antibody (ab245530) at 0.04 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 197 kDa
Exposure time: 75 seconds
Lysates prepared in NETN buffer.
ARID2 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab245530 at 6 µg per reaction. Western blot was performed from the immunoprecipitate using ab245530 at 1 µg/ml.
Lane 1: ab245530 (Batch 2) IP in HeLa whole cell lysate.
Lane 2: ab245530 (Batch 1) IP in HeLa whole cell lysate.
Lane 3: Control IgG IP in HeLa whole cell lysate.
Detection: Chemiluminescence with exposure time of 30 seconds.
Lysates prepared in NETN buffer.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab245530 has not yet been referenced specifically in any publications.