Key features and details
- Rabbit polyclonal to ARID3A - C-terminal
- Suitable for: ICC/IF, IP, IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-ARID3A antibody - C-terminal
See all ARID3A primary antibodies
DescriptionRabbit polyclonal to ARID3A - C-terminal
Tested applicationsSuitable for: ICC/IF, IP, IHC-P, WBmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat
Recombinant fragment within Human ARID3A (C terminal). The exact sequence is proprietary.
Database link: Q99856
- WB:HCT 116 whole cell lysate. IP: HEK-293T whole cell lysate. IHC-P: Mouse lymph node and testis tissue. ICC/IF: HeLa cells.
This product was previously labelled as DRIL1
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab227274 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/100 - 1/1000.|
|IP||1/100 - 1/500.|
|IHC-P||1/100 - 1/1000.|
|WB||1/500 - 1/3000. Predicted molecular weight: 63 kDa.|
FunctionTranscription factor which may be involved in the control of cell cycle progression by the RB1/E2F1 pathway and in B-cell differentiation.
Tissue specificityWidely expressed, with highest expression in skeletal muscle, thalamus, and colon.
Sequence similaritiesContains 1 ARID domain.
Contains 1 REKLES domain.
Cellular localizationNucleus. Cytoplasm. Shuttles between nucleus and cytoplasm.
- Information by UniProt
- ARI3A_HUMAN antibody
- ARID domain containing 3A antibody
- ARID domain-containing protein 3A antibody
Anti-ARID3A antibody - C-terminal (ab227274) at 1/1000 dilution + HCT 116 (human colorectal carcinoma cell line) whole cell lysate at 30 µg
Developed using the ECL technique.
Predicted band size: 63 kDa
7.5% SDS PAGE
Paraffin-embedded mouse testis tissue stained for ARID3A with ab227274 at 1/500 dilution in immunohistochemical analysis.
Paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for ARID3A (green) using ab227274 at 1/500 dilution in ICC/IF.
Blue: Hoechst 33342 staining (lower panel).
ARID3A was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate with 2 μg ab227274. Western blot was performed from the immunoprecipitate using ab227274 at 1/1000 dilution.
Lane 1: HEK-293T whole cell lysate 40 μg (Input).
Lane 2: Control IgG IP in HEK-293T whole cell lysate.
Lane 3: ab227274 IP in HEK-293T whole cell lysate.
Paraffin-embedded mouse lymph node tissue stained for ARID3A with ab227274 at 1/500 dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab227274 has not yet been referenced specifically in any publications.