Product nameAnti-ARID3A antibody - C-terminal
See all ARID3A primary antibodies
DescriptionRabbit polyclonal to ARID3A - C-terminal
Tested applicationsSuitable for: ICC/IF, IP, IHC-P, WBmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat
Recombinant fragment within Human ARID3A (C terminal). The exact sequence is proprietary.
Database link: Q99856
- WB:HCT 116 whole cell lysate. IP: HEK-293T whole cell lysate. IHC-P: Mouse lymph node and testis tissue. ICC/IF: HeLa cells.
This product was previously labelled as DRIL1
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab227274 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/100 - 1/1000.|
|IP||1/100 - 1/500.|
|IHC-P||1/100 - 1/1000.|
|WB||1/500 - 1/3000. Predicted molecular weight: 63 kDa.|
FunctionTranscription factor which may be involved in the control of cell cycle progression by the RB1/E2F1 pathway and in B-cell differentiation.
Tissue specificityWidely expressed, with highest expression in skeletal muscle, thalamus, and colon.
Sequence similaritiesContains 1 ARID domain.
Contains 1 REKLES domain.
Cellular localizationNucleus. Cytoplasm. Shuttles between nucleus and cytoplasm.
- Information by UniProt
- ARI3A_HUMAN antibody
- ARID domain containing 3A antibody
- ARID domain-containing protein 3A antibody
Anti-ARID3A antibody - C-terminal (ab227274) at 1/1000 dilution + HCT 116 (human colorectal carcinoma cell line) whole cell lysate at 30 µg
Developed using the ECL technique.
Predicted band size: 63 kDa
7.5% SDS PAGE
Paraffin-embedded mouse testis tissue stained for ARID3A with ab227274 at 1/500 dilution in immunohistochemical analysis.
Paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for ARID3A (green) using ab227274 at 1/500 dilution in ICC/IF.
Blue: Hoechst 33342 staining (lower panel).
ARID3A was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate with 2 μg ab227274. Western blot was performed from the immunoprecipitate using ab227274 at 1/1000 dilution.
Lane 1: HEK-293T whole cell lysate 40 μg (Input).
Lane 2: Control IgG IP in HEK-293T whole cell lysate.
Lane 3: ab227274 IP in HEK-293T whole cell lysate.
Paraffin-embedded mouse lymph node tissue stained for ARID3A with ab227274 at 1/500 dilution in immunohistochemical analysis.
ab227274 has not yet been referenced specifically in any publications.