Overview

  • Product name

    Anti-ARID3A antibody - C-terminal
    See all ARID3A primary antibodies
  • Description

    Rabbit polyclonal to ARID3A - C-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, IP, IHC-P, WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Recombinant fragment within Human ARID3A (C terminal). The exact sequence is proprietary.
    Database link: Q99856

  • Positive control

    • WB:HCT 116 whole cell lysate. IP: HEK-293T whole cell lysate. IHC-P: Mouse lymph node and testis tissue. ICC/IF: HeLa cells.
  • General notes

     This product was previously labelled as DRIL1

     

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab227274 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/1000.
IP 1/100 - 1/500.
IHC-P 1/100 - 1/1000.
WB 1/500 - 1/3000. Predicted molecular weight: 63 kDa.

Target

  • Function

    Transcription factor which may be involved in the control of cell cycle progression by the RB1/E2F1 pathway and in B-cell differentiation.
  • Tissue specificity

    Widely expressed, with highest expression in skeletal muscle, thalamus, and colon.
  • Sequence similarities

    Contains 1 ARID domain.
    Contains 1 REKLES domain.
  • Cellular localization

    Nucleus. Cytoplasm. Shuttles between nucleus and cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • ARI3A_HUMAN antibody
    • ARID domain containing 3A antibody
    • ARID domain-containing protein 3A antibody
    • ARID3A antibody
    • AT rich interactive domain 3A (BRIGHT- like) protein antibody
    • AT rich interactive domain 3A (BRIGHT-like) antibody
    • AT rich interactive domain-containing protein 3A antibody
    • AT-rich interactive domain-containing protein 3A antibody
    • B cell regulator of IgH transcription antibody
    • B-cell regulator of IgH transcription antibody
    • Bright antibody
    • dead ringer like 1 antibody
    • dead ringer, Drosophila, homolog-like 1 antibody
    • Dead ringer-like protein 1 antibody
    • DRIL1 antibody
    • DRIL3 antibody
    • DRX antibody
    • E2F binding protein 1 antibody
    • E2F-binding protein 1 antibody
    • E2FBP1 antibody
    • Homo sapiens AT rich interactive domain 3A (BRIGHT-like) antibody
    see all

Images

  • Anti-ARID3A antibody - C-terminal (ab227274) at 1/1000 dilution + HCT 116 (human colorectal carcinoma cell line) whole cell lysate at 30 µg

    Developed using the ECL technique.

    Predicted band size: 63 kDa



    7.5% SDS PAGE

  • Paraffin-embedded mouse testis tissue stained for ARID3A with ab227274 at 1/500 dilution in immunohistochemical analysis.

  • Paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for ARID3A (green) using ab227274 at 1/500 dilution in ICC/IF.

    Blue: Hoechst 33342 staining (lower panel).

  • ARID3A was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate with 2 μg ab227274. Western blot was performed from the immunoprecipitate using ab227274 at 1/1000 dilution.  

    Lane 1: HEK-293T whole cell lysate 40 μg (Input).

    Lane 2: Control IgG IP in HEK-293T whole cell lysate.

    Lane 3: ab227274 IP in HEK-293T whole cell lysate.

    7.5% SDS-PAGE

  • Paraffin-embedded mouse lymph node tissue stained for ARID3A with ab227274 at 1/500 dilution in immunohistochemical analysis.

References

ab227274 has not yet been referenced specifically in any publications.

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