Overview

  • Product name

    Anti-ARID3A antibody [EPR11288]
    See all ARID3A primary antibodies
  • Description

    Rabbit monoclonal [EPR11288] to ARID3A
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, IHC-P, WBmore details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    corresponding to Human ARID3A aa 450-550 (Cysteine residue).
    Database link: Q99856

  • Positive control

    • Human colon, skeletal muscle, placenta, fetal brain and fetal heart lysates; Human ovarian carcinoma and spleen tissues; HepG2 cells.
  • General notes

     

     

     This product was previously labelled as DRIL1

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR11288
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab169787 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/250 - 1/500.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. The use of an HRP/AP polymerized secondary antibody is recommended.
WB 1/1000 - 1/5000. Predicted molecular weight: 63 kDa.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Function

      Transcription factor which may be involved in the control of cell cycle progression by the RB1/E2F1 pathway and in B-cell differentiation.
    • Tissue specificity

      Widely expressed, with highest expression in skeletal muscle, thalamus, and colon.
    • Sequence similarities

      Contains 1 ARID domain.
      Contains 1 REKLES domain.
    • Cellular localization

      Nucleus. Cytoplasm. Shuttles between nucleus and cytoplasm.
    • Information by UniProt
    • Database links

    • Alternative names

      • ARI3A_HUMAN antibody
      • ARID domain containing 3A antibody
      • ARID domain-containing protein 3A antibody
      • ARID3A antibody
      • AT rich interactive domain 3A (BRIGHT- like) protein antibody
      • AT rich interactive domain 3A (BRIGHT-like) antibody
      • AT rich interactive domain-containing protein 3A antibody
      • AT-rich interactive domain-containing protein 3A antibody
      • B cell regulator of IgH transcription antibody
      • B-cell regulator of IgH transcription antibody
      • Bright antibody
      • dead ringer like 1 antibody
      • dead ringer, Drosophila, homolog-like 1 antibody
      • Dead ringer-like protein 1 antibody
      • DRIL1 antibody
      • DRIL3 antibody
      • DRX antibody
      • E2F binding protein 1 antibody
      • E2F-binding protein 1 antibody
      • E2FBP1 antibody
      • Homo sapiens AT rich interactive domain 3A (BRIGHT-like) antibody
      see all

    Images

    • All lanes : Anti-ARID3A antibody [EPR11288] (ab169787) at 1/1000 dilution

      Lane 1 : Human colon lysate
      Lane 2 : Human fetal brain lysate
      Lane 3 : Human skeletal muscle lysate
      Lane 4 : Human fetal heart lysate
      Lane 5 : Human placenta lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Developed using the ECL technique.

      Predicted band size: 63 kDa

    • Immunofluorescent analysis of HepG2 cells labeling ARID3A with ab169787 at 1/250 dilution.

    • Immunohistochemical analysis of paraffin-embedded, formalin-fixed Human ovarian carcinoma tissue labeling ARID3A with ab169787 at 1/50 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemical analysis of paraffin-embedded, formalin-fixed Human spleen tissue labeling ARID3A with ab169787 at 1/50 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    References

    ab169787 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab169787.
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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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