Anti-ARID5A antibody (ab151449)
Key features and details
- Rabbit polyclonal to ARID5A
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-ARID5A antibody -
Description
Rabbit polyclonal to ARID5A -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Cow -
Immunogen
Recombinant fragment corresponding to a region within amino acids 11 and 259 of Human ARID5A (UniProt ID: Q03989).
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Positive control
- 293T, A431 and HeLa cell lysates; 59T xenograft tissue
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab151449 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 64 kDa.
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IHC-P |
1/100 - 1/1000.
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Notes |
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WB
1/1000 - 1/10000. Predicted molecular weight: 64 kDa. |
IHC-P
1/100 - 1/1000. |
Target
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Relevance
ARID5A is a member of the ARID protein family, that have diverse functions but all appear to play important roles in development, tissue-specific gene expression, and regulation of cell growth. -
Cellular localization
Nuclear -
Database links
- Entrez Gene: 10865 Human
- Omim: 611583 Human
- SwissProt: Q03989 Human
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Alternative names
- ARID domain containing protein 5A antibody
- ARID-containing protein 5A antibody
- AT rich interactive domain 5A antibody
see all
Images
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All lanes : Anti-ARID5A antibody (ab151449) at 1/5000 dilution
Lane 1 : 293T whole cell lysate
Lane 2 : A431 whole cell lysate
Lane 3 : HeLa whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 64 kDa
7.5% SDS PAGE -
Immunohistochemical analysis of paraffin-embedded 59T xenograft tissue labeling ARID5A with ab151449 at 1/500 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab151449 has been referenced in 2 publications.
- Ikeuchi W et al. AT-rich interaction domain 5A regulates the transcription of interleukin-6 gene in prostate cancer cells. Prostate 82:97-106 (2022). PubMed: 34633095
- Anwar T et al. Differentially regulated gene expression in quiescence versus senescence and identification of ARID5A as a quiescence associated marker. J Cell Physiol 233:3695-3712 (2018). PubMed: 29044508