Key features and details
- Rabbit polyclonal to ARID5A
- Suitable for: WB, ELISA, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-ARID5A antibody
See all ARID5A primary antibodies
DescriptionRabbit polyclonal to ARID5A
Tested applicationsSuitable for: WB, ELISA, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cat, Dog, Pig
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 2% Sucrose, PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab81149 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 22, 34, 64 kDa (predicted molecular weight: 64 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|
|ELISA||Use at an assay dependent concentration.
ELISA titre using peptide based assay 1/62500.
|IHC-P||Use a concentration of 5 µg/ml.|
RelevanceARID5A is a member of the ARID protein family, that have diverse functions but all appear to play important roles in development, tissue-specific gene expression, and regulation of cell growth.
- ARID domain containing protein 5A antibody
- ARID-containing protein 5A antibody
- AT rich interactive domain 5A antibody
Anti-ARID5A antibody (ab81149) at 1 µg/ml + 721_B cell lysate at 10 µg
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size: 64 kDa
Observed band size: 64 kDa
Additional bands at: <22 kDa, 34 kDa. We are unsure as to the identity of these extra bands.
Gel concentration 12%
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate tissue labelling ARID5A with ab81149 at 5.0µg/ml.
ab81149 has been referenced in 1 publication.
- Nyati KK et al. TLR4-induced NF-?B and MAPK signaling regulate the IL-6 mRNA stabilizing protein Arid5a. Nucleic Acids Res 45:2687-2703 (2017). WB . PubMed: 28168301