Overview

  • Product name

  • Description

    Rabbit polyclonal to ARID5B
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human ARID5B (internal sequence). The exact sequence is proprietary.
    Database link: Q14865

  • Positive control

    • WB: HeLa whole cell and nuclear extracts.

Properties

Applications

Our Abpromise guarantee covers the use of ab226776 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 36,105,132 kDa.

Target

  • Function

    Transcription coactivator that binds to the 5'-AATA[CT]-3' core sequence and plays a key role in adipogenesis and liver development. Acts by forming a complex with phosphorylated PHF2, which mediates demethylation at Lys-336, leading to target the PHF2-ARID5B complex to target promoters, where PHF2 mediates demethylation of dimethylated 'Lys-9' of histone H3 (H3K9me2), followed by transcription activation of target genes. The PHF2-ARID5B complex acts as a coactivator of HNF4A in liver. Required for adipogenesis: regulates triglyceride metabolism in adipocytes by regulating expression of adipogenic genes. Overexpression leads to induction of smooth muscle marker genes, suggesting that it may also act as a regulator of smooth muscle cell differentiation and proliferation. Represses the cytomegalovirus enhancer.
  • Tissue specificity

    Widely expressed, including in liver (at protein level).
  • Involvement in disease

    Note=Defects in ARID5B ay be a cause of susceptibility to coronary atherosclerosis in the Japanese population.
    Defects in ARID5B may be a cause of susceptibility to acute lymphoblastic leukemia (ALL) [MIM:613065]. Note=ALL is a subtype of acute leukemia, a cancer of the white blood cells. ALL is a malignant disease of bone marrow and the most common malignancy diagnosed in children. The malignant cells are lymphoid precursor cells (lymphoblasts) that are arrested in an early stage of development. The lymphoblasts replace the normal marrow elements, resulting in a marked decrease in the production of normal blood cells. Consequently, anemia, thrombocytopenia, and neutropenia occur to varying degrees. The lymphoblasts also proliferate in organs other than the marrow, particularly the liver, spleen, and lymphonodes.
  • Sequence similarities

    Belongs to the ARID5B family.
    Contains 1 ARID domain.
  • Domain

    The ARID domain mediates the interaction with DNA.
  • Post-translational
    modifications

    Methylation at Lys-336 prevents DNA-binding. Demethylation by PHF2 promotes recruitment of the PHF2-ARID5B complex to promoters.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • ARI5B_HUMAN antibody
    • ARID domain-containing protein 5B antibody
    • Arid5b antibody
    • AT rich interactive domain 5B (MRF1 like) antibody
    • AT-rich interactive domain-containing protein 5B antibody
    • DESRT antibody
    • FLJ21150 antibody
    • FLJ41888 antibody
    • Modulator recognition factor 2 (MRF2) antibody
    • Modulator recognition factor 2 antibody
    • MRF-2 antibody
    • Mrf1-like antibody
    • MRF1-like protein antibody
    • MRF2 antibody
    • OTTHUMP00000019668 antibody
    • RP11 341A19.1 antibody
    see all

Images

  • All lanes : Anti-ARID5B antibody (ab226776) at 1/500 dilution

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
    Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) nuclear extract

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 36,105,132 kDa



    5% SDS-PAGE gel.

References

This product has been referenced in:

  • Ge Z  et al. Aberrant ARID5B expression and its association with Ikaros dysfunction in acute lymphoblastic leukemia. Oncogenesis 7:84 (2018). Read more (PubMed: 30420689) »
See 1 Publication for this product

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