Overview

  • Product name

    Anti-ARIH2/TRIAD1 antibody
    See all ARIH2/TRIAD1 primary antibodies
  • Description

    Goat polyclonal to ARIH2/TRIAD1
  • Host species

    Goat
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Cow, Dog, Xenopus laevis, Zebrafish
  • Immunogen

    Synthetic peptide corresponding to Human ARIH2/TRIAD1 aa 480-493 (C terminal).
    Sequence:

    EQRRRTLLKDFHDT

  • General notes

     This product was previously labelled as ARIH2

     

Properties

Applications

Our Abpromise guarantee covers the use of ab17014 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 3 µg/ml.

Approx 60kDa band observed in nuclear lysates of Hela and K562 (Calculated MW of 57.8kDa according to NP_006312.1). Customer's experiments gave a band at approx 60kDa in lysates in both Human HeLa and Mouse Muscle (IM2 & C2C12) cells. This band disappeared after RNAi knock-down.

ICC/IF Use at an assay dependent concentration.

Target

  • Function

    E3 ubiquitin-protein ligase mediating 'Lys-48'-and 'Lys-63'-linked polyubiquitination and subsequent proteasomal degradation of modified proteins. May play a role in myelopoiesis.
  • Tissue specificity

    Widely expressed with higher expression in granulocytes.
  • Pathway

    Protein modification; protein ubiquitination.
  • Sequence similarities

    Belongs to the RBR family. Ariadne subfamily.
    Contains 1 IBR-type zinc finger.
    Contains 2 RING-type zinc fingers.
  • Domain

    RING-type 1 and RING-type 2 are required for the inhibitory function in myelopoiesis.
  • Post-translational
    modifications

    Ubiquitinated. Ubiquitination promotes proteasomal degradation.
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization

    Nucleus. Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • HT005 antibody
    • all trans retinoic acid inducible RING finger antibody
    • Ari 2 antibody
    • Ari-2 antibody
    • ARI2 antibody
    • ARI2_HUMAN antibody
    • Ariadne 2 antibody
    • Ariadne 2 protein homolog antibody
    • ariadne homolog 2 (Drosophila) antibody
    • ariadne homolog 2 antibody
    • Ariadne RBR E3 ubiquitin protein ligase 2 antibody
    • ARIH 2 antibody
    • Arih2 antibody
    • E3 ubiquitin protein ligase ARIH2 antibody
    • E3 ubiquitin-protein ligase ARIH2 antibody
    • protein ariadne 2 homolog antibody
    • Protein ariadne-2 homolog antibody
    • Triad 1 antibody
    • TRIAD1 antibody
    • Triad1 protein antibody
    • UIP48 antibody
    see all

Images

  • All lanes : Anti-ARIH2/TRIAD1 antibody (ab17014) at 2 µg/ml

    Lane 1 : HeLa nuclear lysate
    Lane 2 : K562 nuclear lysate

    Lysates/proteins at 35 µg per lane.

References

This product has been referenced in:

See 1 Publication for this product

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab17014.
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