Key features and details
- Rabbit polyclonal to ARIH2/TRIAD1
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-ARIH2/TRIAD1 antibody
See all ARIH2/TRIAD1 primary antibodies
DescriptionRabbit polyclonal to ARIH2/TRIAD1
Tested applicationsSuitable for: ICC/IF, WBmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Cow, Chimpanzee
Recombinant fragment within Human ARIH2/TRIAD1 (internal sequence). The exact sequence is proprietary.
Database link: O95376
- ICC/IF: HeLa cells. WB: HL-60 cell lysate; mouse leukocyte lysate.
This product was previously labelled as ARIH2
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 79.99% PBS, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab228698 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/100 - 1/1000.|
|WB||1/500 - 1/3000. Predicted molecular weight: 57 kDa.|
FunctionE3 ubiquitin-protein ligase mediating 'Lys-48'-and 'Lys-63'-linked polyubiquitination and subsequent proteasomal degradation of modified proteins. May play a role in myelopoiesis.
Tissue specificityWidely expressed with higher expression in granulocytes.
PathwayProtein modification; protein ubiquitination.
Sequence similaritiesBelongs to the RBR family. Ariadne subfamily.
Contains 1 IBR-type zinc finger.
Contains 2 RING-type zinc fingers.
DomainRING-type 1 and RING-type 2 are required for the inhibitory function in myelopoiesis.
modificationsUbiquitinated. Ubiquitination promotes proteasomal degradation.
Phosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localizationNucleus. Cytoplasm.
- Information by UniProt
- HT005 antibody
- all trans retinoic acid inducible RING finger antibody
- Ari 2 antibody
HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for ARIH2/TRIAD1 (green) using ab228698 at 1/500 dilution in ICC/IF. Cells were fixed in 4% paraformaldehyde/PBS for 15 minutes.
Nuclear counterstain: Hoechst 33342 (blue).
Anti-ARIH2/TRIAD1 antibody (ab228698) at 1/1000 dilution + Mouse leukocyte lysate at 50 µg
HRP-conjugated anti-rabbit IgG
Predicted band size: 57 kDa
Anti-ARIH2/TRIAD1 antibody (ab228698) at 1/1000 dilution + HL-60 (human promyelocytic leukemia cell line) cell lysate at 30 µg
Predicted band size: 57 kDa
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab228698 has not yet been referenced specifically in any publications.