Overview

  • Product name

    Anti-ARIH2/TRIAD1 antibody
    See all ARIH2/TRIAD1 primary antibodies
  • Description

    Rabbit polyclonal to ARIH2/TRIAD1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Cow, Chimpanzee
  • Immunogen

    Recombinant fragment within Human ARIH2/TRIAD1 (internal sequence). The exact sequence is proprietary.
    Database link: O95376

  • Positive control

    • ICC/IF: HeLa cells. WB: HL-60 cell lysate; mouse leukocyte lysate.
  • General notes

     This product was previously labelled as ARIH2

     

Properties

Applications

Our Abpromise guarantee covers the use of ab228698 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/1000.
WB 1/500 - 1/3000. Predicted molecular weight: 57 kDa.

Target

  • Function

    E3 ubiquitin-protein ligase mediating 'Lys-48'-and 'Lys-63'-linked polyubiquitination and subsequent proteasomal degradation of modified proteins. May play a role in myelopoiesis.
  • Tissue specificity

    Widely expressed with higher expression in granulocytes.
  • Pathway

    Protein modification; protein ubiquitination.
  • Sequence similarities

    Belongs to the RBR family. Ariadne subfamily.
    Contains 1 IBR-type zinc finger.
    Contains 2 RING-type zinc fingers.
  • Domain

    RING-type 1 and RING-type 2 are required for the inhibitory function in myelopoiesis.
  • Post-translational
    modifications

    Ubiquitinated. Ubiquitination promotes proteasomal degradation.
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization

    Nucleus. Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • HT005 antibody
    • all trans retinoic acid inducible RING finger antibody
    • Ari 2 antibody
    • Ari-2 antibody
    • ARI2 antibody
    • ARI2_HUMAN antibody
    • Ariadne 2 antibody
    • Ariadne 2 protein homolog antibody
    • ariadne homolog 2 (Drosophila) antibody
    • ariadne homolog 2 antibody
    • Ariadne RBR E3 ubiquitin protein ligase 2 antibody
    • ARIH 2 antibody
    • Arih2 antibody
    • E3 ubiquitin protein ligase ARIH2 antibody
    • E3 ubiquitin-protein ligase ARIH2 antibody
    • protein ariadne 2 homolog antibody
    • Protein ariadne-2 homolog antibody
    • Triad 1 antibody
    • TRIAD1 antibody
    • Triad1 protein antibody
    • UIP48 antibody
    see all

Images

  • HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for ARIH2/TRIAD1 (green) using ab228698 at 1/500 dilution in ICC/IF. Cells were fixed in 4% paraformaldehyde/PBS for 15 minutes.

    Nuclear counterstain: Hoechst 33342 (blue).

  • Anti-ARIH2/TRIAD1 antibody (ab228698) at 1/1000 dilution + Mouse leukocyte lysate at 50 µg

    Secondary
    HRP-conjugated anti-rabbit IgG

    Predicted band size: 57 kDa

  • Anti-ARIH2/TRIAD1 antibody (ab228698) at 1/1000 dilution + HL-60 (human promyelocytic leukemia cell line) cell lysate at 30 µg

    Predicted band size: 57 kDa

References

ab228698 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab228698.
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