Overview

  • Product name

    Anti-ARIH2/TRIAD1 antibody [EPR7670]
    See all ARIH2/TRIAD1 primary antibodies
  • Description

    Rabbit monoclonal [EPR7670] to ARIH2/TRIAD1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, Flow Cytmore details
    Unsuitable for: ICC,IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human ARIH2/TRIAD1 aa 100-200. The exact sequence is proprietary.

  • Positive control

    • Jurkat, K562, 293T cell lysate; K562 cells
  • General notes

     

     

     This product was previously labelled as ARIH2

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab133744 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 58 kDa (predicted molecular weight: 57 kDa).
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for ICC,IHC-P or IP.
  • Target

    • Function

      E3 ubiquitin-protein ligase mediating 'Lys-48'-and 'Lys-63'-linked polyubiquitination and subsequent proteasomal degradation of modified proteins. May play a role in myelopoiesis.
    • Tissue specificity

      Widely expressed with higher expression in granulocytes.
    • Pathway

      Protein modification; protein ubiquitination.
    • Sequence similarities

      Belongs to the RBR family. Ariadne subfamily.
      Contains 1 IBR-type zinc finger.
      Contains 2 RING-type zinc fingers.
    • Domain

      RING-type 1 and RING-type 2 are required for the inhibitory function in myelopoiesis.
    • Post-translational
      modifications

      Ubiquitinated. Ubiquitination promotes proteasomal degradation.
      Phosphorylated upon DNA damage, probably by ATM or ATR.
    • Cellular localization

      Nucleus. Cytoplasm.
    • Information by UniProt
    • Database links

    • Alternative names

      • HT005 antibody
      • all trans retinoic acid inducible RING finger antibody
      • Ari 2 antibody
      • Ari-2 antibody
      • ARI2 antibody
      • ARI2_HUMAN antibody
      • Ariadne 2 antibody
      • Ariadne 2 protein homolog antibody
      • ariadne homolog 2 (Drosophila) antibody
      • ariadne homolog 2 antibody
      • Ariadne RBR E3 ubiquitin protein ligase 2 antibody
      • ARIH 2 antibody
      • Arih2 antibody
      • E3 ubiquitin protein ligase ARIH2 antibody
      • E3 ubiquitin-protein ligase ARIH2 antibody
      • protein ariadne 2 homolog antibody
      • Protein ariadne-2 homolog antibody
      • Triad 1 antibody
      • TRIAD1 antibody
      • Triad1 protein antibody
      • UIP48 antibody
      see all

    Images

    • All lanes : Anti-ARIH2/TRIAD1 antibody [EPR7670] (ab133744) at 1/1000 dilution

      Lane 1 : Jurkat cell lysate
      Lane 2 : K562 cell lysate
      Lane 3 : 293T cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-Rabbit HRP at 1/2000 dilution

      Predicted band size: 57 kDa
      Observed band size: 58 kDa
      why is the actual band size different from the predicted?

    • Flow cytometrical analysis of permeabilized K562 cells labelling ARIH2/TRIAD1 with ab133744 at 1/10 (red) with rabbit IgG negative control (green).

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    ab133744 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab133744.
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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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