Product nameAnti-ARIH2/TRIAD1 antibody [EPR7671]
See all ARIH2/TRIAD1 primary antibodies
DescriptionRabbit monoclonal [EPR7671] to ARIH2/TRIAD1
Tested applicationsSuitable for: WB, ICC, IPmore details
Unsuitable for: Flow Cyt or IHC-P
Species reactivityReacts with: Mouse, Rat, Human
corresponding to Human ARIH2/TRIAD1 aa 400-500.
Database link: O95376
- 293T, Jurkat, HeLa and PC12 cell lysates
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Dissociation constant (KD)KD = 9.00 x 10 -12 M Learn more about KD
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab133653 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 57 kDa.|
|ICC||1/100 - 1/250.|
|IP||1/10 - 1/100.|
FunctionE3 ubiquitin-protein ligase mediating 'Lys-48'-and 'Lys-63'-linked polyubiquitination and subsequent proteasomal degradation of modified proteins. May play a role in myelopoiesis.
Tissue specificityWidely expressed with higher expression in granulocytes.
PathwayProtein modification; protein ubiquitination.
Sequence similaritiesBelongs to the RBR family. Ariadne subfamily.
Contains 1 IBR-type zinc finger.
Contains 2 RING-type zinc fingers.
DomainRING-type 1 and RING-type 2 are required for the inhibitory function in myelopoiesis.
modificationsUbiquitinated. Ubiquitination promotes proteasomal degradation.
Phosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localizationNucleus. Cytoplasm.
- Information by UniProt
- HT005 antibody
- all trans retinoic acid inducible RING finger antibody
- Ari 2 antibody
All lanes : Anti-ARIH2/TRIAD1 antibody [EPR7671] (ab133653) at 1/1000 dilution
Lane 1 : 293T cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : PC12 cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 57 kDa
Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD
ab133653 has not yet been referenced specifically in any publications.