Overview

  • Product name

  • Description

    Rabbit polyclonal to ARK5
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ELISA, WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Hamster, Human
  • Immunogen

    Synthetic peptide conjugated to KLH, corresponding to sequence from within N terminal amino acids 1-100 of Human ARK5.

  • Positive control

    • Mouse brain tissue lysate, Human breast carcinoma.

Properties

Applications

Our Abpromise guarantee covers the use of ab37641 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/1000.
WB 1/100 - 1/500. Detects a band of approximately 74 kDa (predicted molecular weight: 74 kDa).
IHC-P Use at an assay dependent concentration.

Target

  • Function

    Involved in tolerance to glucose starvation. Phosphorylates ATM. Suppresses Fas-induced apoptosis by phosphorylation of CASP6, thus suppressing the activation of the caspase and the subsequent cleavage of CFLAR.
  • Tissue specificity

    Expressed at high levels in heart and brain, and at lower levels in skeletal muscle, kidney, ovary, placenta, lung and liver.
  • Sequence similarities

    Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications

    Ubiquitinated with 'Lys-29'- and 'Lys-33'-linked polyubiquitins which appear to impede LKB1-mediated phosphorylation. Deubiquitinated by USP9X.
  • Information by UniProt
  • Database links

  • Alternative names

    • AMP activated protein kinase family member 5 antibody
    • AMPK related protein kinase 5 antibody
    • AMPK-related protein kinase 5 antibody
    • NUAK family SNF1 like kinase antibody
    • NUAK family SNF1-like kinase 1 antibody
    • Nuak1 antibody
    • NUAK1_HUMAN antibody
    • Omphalocele kinase 1 antibody
    • Probable serine/threonine protein kinase KIAA0537 antibody
    see all

Images

  • Anti-ARK5 antibody (ab37641) at 1/100 dilution + Mouse brain tissue lysate

    Predicted band size: 74 kDa
    Observed band size: 74 kDa

  • ab37641, at 1/50 dilution, staining ARK5 in Human breast carcinoma by Immunohistochemistry, Formalin fixed, Paraffin embedded tissue. ab37641 was peroxidase-conjugated to the secondary antibody, followed by AEC staining.

References

This product has been referenced in:

  • Wang C  et al. Upregulation of lncRNA snoRNA host gene 6 regulates NUAK family SnF1-like kinase-1 expression by competitively binding microRNA-125b and interacting with Snail1/2 in bladder cancer. J Cell Biochem 120:357-367 (2019). Read more (PubMed: 30168179) »
  • Peng JK  et al. ?ypoxia-inducible factor 1-a promotes colon cell proliferation and migration by upregulating AMPK-related protein kinase 5 under hypoxic conditions. Oncol Lett 15:3639-3645 (2018). Read more (PubMed: 29467884) »
See all 2 Publications for this product

Customer reviews and Q&As

Application
Western blot
Loading amount
25 µg
Gel Running Conditions
Reduced Denaturing (10%)
Sample
Human Cell lysate - whole cell (HEK293)
Specification
HEK293
Blocking step
Odyssey block as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 20°C

Abcam user community

Verified customer

Submitted Apr 24 2014

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