Recombinant
RabMAb

Recombinant Anti-ARL 1 antibody [EPR10595] (ab155982)

Overview

  • Product name

    Anti-ARL 1 antibody [EPR10595]
    See all ARL 1 primary antibodies
  • Description

    Rabbit monoclonal [EPR10595] to ARL 1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, IP, ICC/IFmore details
    Unsuitable for: Flow Cyt
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide, corresponding to residues in Human ARL 1 (UniProt: P40616).

  • Positive control

    • HepG2, MCF7, HeLa and K562 cell lysate; Human liver and pancreas tissues. This antibody gave a positive result when used in the following formaldehyde fixed cell lines: CaCo2
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20ºC.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR10595
  • Isotype

    IgG

Applications

Our Abpromise guarantee covers the use of ab155982 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 20 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IP 1/10 - 1/100.
ICC/IF 1/100 - 1/250.
  • Application notes
    Is unsuitable for Flow Cyt.
  • Target

    • Function

      GTP-binding protein that has very low efficiency as allosteric activator of the cholera toxin catalytic subunit, an ADP-ribosyltransferase. Can activate phospholipase D with very low efficiency. Important for normal function of the Golgi apparatus.
    • Tissue specificity

      Detected in heart, liver, lung and liver (at protein level). Detected in fetal heart, lung, liver and kidney. Detected in adult heart, placenta, lung, liver, skeletal muscle, kidney and pancreas.
    • Sequence similarities

      Belongs to the small GTPase superfamily. Arf family.
    • Cellular localization

      Golgi apparatus membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • ADP ribosylation factor like 1 antibody
      • ADP-ribosylation factor-like protein 1 antibody
      • ARFL1 antibody
      • Arl1 antibody
      • ARL1_HUMAN antibody
      see all

    Images

    • All lanes : Anti-ARL 1 antibody [EPR10595] (ab155982) at 1/1000 dilution

      Lane 1 : HepG2 cell lysate
      Lane 2 : MCF7 cell lysate
      Lane 3 : HeLa cell lysate
      Lane 4 : K562 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labeled goat anti-rabbit IgG at 1/2000 dilution

      Predicted band size: 20 kDa

    • ICC/IF image of ab155982 stained CaCo2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab155982 at 10µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    • ab155982 showing +ve staining in Human normal prostate.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • ab155982 showing +ve staining in Human gastric adenocarcinoma.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • ab155982 showing +ve staining in Human endometrial carcinoma.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • ab155982 showing +ve staining in Human normal brain.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • ab155982 showing +ve staining in Human normal liver.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling ARL 1 with ab155982 at 1/50 dilution.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human pancreas tissue labeling ARL 1 with ab155982 at 1/50 dilution

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    References

    ab155982 has not yet been referenced specifically in any publications.

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