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    arl13b-antibody-ab153725.pdf

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Signal Transduction Signaling Pathway G Protein Signaling Small G Proteins Ras Family
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Anti-ARL13B antibody (ab153725)

  • Datasheet
  • SDS
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Western blot - Anti-ARL13B antibody (ab153725)
  • Western blot - Anti-ARL13B antibody (ab153725)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARL13B antibody (ab153725)

Key features and details

  • Rabbit polyclonal to ARL13B
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-ARL13B antibody
    See all ARL13B primary antibodies
  • Description

    Rabbit polyclonal to ARL13B
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    A recombinant fragment corresponding to a region within amino acids 141 and 428 of Human ARL13B

  • Positive control

    • 293T cell lysate; Mouse liver tissue lysate; Paraffin-embedded U87 xenograft
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Signaling Pathway
    • G Protein Signaling
    • Small G Proteins
    • Ras Family

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • Mouse liver tissue lysate - total protein (ab29301)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab153725 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/500 - 1/3000. Predicted molecular weight: 48 kDa.
IHC-P
1/100 - 1/1000.
Notes
WB
1/500 - 1/3000. Predicted molecular weight: 48 kDa.
IHC-P
1/100 - 1/1000.

Target

  • Involvement in disease

    Defects in ARL13B are the cause of Joubert syndrome type 8 (JBTS8) [MIM:612291]. JBTS is an autosomal recessive disorder presenting with cerebellar ataxia, oculomotor apraxia, hypotonia, neonatal breathing abnormalities and psychomotor delay. Neuroradiologically, it is characterized by cerebellar vermis hypoplasia/aplasia, thickened and reoriented superior cerebellar peduncles, and an abnormally large interpeduncular fossa, giving the appearance of a molar tooth on transaxial slices (molar tooth sign). Additional variable features include retinal dystrophy and renal disease.
  • Sequence similarities

    Belongs to the small GTPase superfamily. Arf family.
  • Target information above from: UniProt accession Q3SXY8 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 200894 Human
    • Entrez Gene: 68146 Mouse
    • Omim: 608922 Human
    • SwissProt: Q3SXY8 Human
    • SwissProt: Q640N2 Mouse
    • Unigene: 533086 Human
    • Unigene: 96833 Mouse
    • Alternative names

      • ADP ribosylation factor like 13B antibody
      • ADP ribosylation factor like 2 like 1 antibody
      • ADP-ribosylation factor-like protein 13B antibody
      • ADP-ribosylation factor-like protein 2-like 1 antibody
      • AR13B_HUMAN antibody
      • arl13b antibody
      • ARL2-like protein 1 antibody
      • ARL2L1 antibody
      • JBTS8 antibody
      see all

    Images

    • Western blot - Anti-ARL13B antibody (ab153725)
      Western blot - Anti-ARL13B antibody (ab153725)
      Anti-ARL13B antibody (ab153725) at 1/1000 dilution + 293T cell lysate at 30 µg

      Predicted band size: 48 kDa



      10% SDS PAGE
    • Western blot - Anti-ARL13B antibody (ab153725)
      Western blot - Anti-ARL13B antibody (ab153725)
      Anti-ARL13B antibody (ab153725) at 1/1000 dilution + Mouse liver tissue lysate at 50 µg

      Predicted band size: 48 kDa



      10% SDS PAGE
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARL13B antibody (ab153725)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARL13B antibody (ab153725)
      Immunohistochemical analysis of paraffin-embedded U87 xenograft, labeling ARL13B using ab153725 at 1/500 dilution.

    Protocols

    • Western blot protocols
    • Immunohistochemistry protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (0)

    Publishing research using ab153725? Please let us know so that we can cite the reference in this datasheet.

    ab153725 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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