Anti-ARL13B antibody [N295B/66] (ab136648)
Key features and details
- Mouse monoclonal [N295B/66] to ARL13B
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat
- Isotype: IgG2a
Overview
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Product name
Anti-ARL13B antibody [N295B/66]
See all ARL13B primary antibodies -
Description
Mouse monoclonal [N295B/66] to ARL13B -
Host species
Mouse -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat -
Immunogen
corresponding to ARL13B.
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Positive control
- WB: mouse fetus tissue lysate, MEF1 whole cell lysate. IHC-P: rat normal brain (choroid plexus) FFPE tissue.
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
N295B/66 -
Myeloma
Sp2/0 -
Isotype
IgG2a -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab136648 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 5 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 60 kDa).
For blocking, we recommend using 1% milk for 1 hour. |
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IHC-P |
Use a concentration of 0.5 - 1 µg/ml.
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Notes |
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WB
Use a concentration of 5 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 60 kDa). For blocking, we recommend using 1% milk for 1 hour. |
IHC-P
Use a concentration of 0.5 - 1 µg/ml. |
Target
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Involvement in disease
Defects in ARL13B are the cause of Joubert syndrome type 8 (JBTS8) [MIM:612291]. JBTS is an autosomal recessive disorder presenting with cerebellar ataxia, oculomotor apraxia, hypotonia, neonatal breathing abnormalities and psychomotor delay. Neuroradiologically, it is characterized by cerebellar vermis hypoplasia/aplasia, thickened and reoriented superior cerebellar peduncles, and an abnormally large interpeduncular fossa, giving the appearance of a molar tooth on transaxial slices (molar tooth sign). Additional variable features include retinal dystrophy and renal disease. -
Sequence similarities
Belongs to the small GTPase superfamily. Arf family. - Information by UniProt
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Database links
- Entrez Gene: 68146 Mouse
- Entrez Gene: 304037 Rat
- SwissProt: Q640N2 Mouse
- Unigene: 96833 Mouse
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Alternative names
- ADP ribosylation factor like 13B antibody
- ADP ribosylation factor like 2 like 1 antibody
- ADP-ribosylation factor-like protein 13B antibody
see all
Images
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IHC image of ARL13B staining in Rat normal brain formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab136648, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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All lanes : Anti-ARL13B antibody [N295B/66] (ab136648) at 5 µg/ml
Lane 1 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lane 2 : Mouse Fetus (14 Day Old) Tissue Lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 60 kDa
Observed band size: 60 kDaThis blot was produced using a 10% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 1 hour 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 1% Milk before being incubated with ab136648 overnight at 4°C. Antibody binding was detected using an anti-mouse antibody conjugated to HRP, and visualised using ECL development solution.
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Anti-ARL13B antibody [N295B/66] (ab136648) at 5 µg/ml + MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 25 µg
Secondary
Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 60 kDa
Observed band size: 60 kDa
Exposure time: 20 minutesThis blot was produced using a 10% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 1% Milk before being incubated with ab136648 overnight at 4°C. Antibody binding was detected using an anti-mouse antibody conjugated to HRP, and visualised using ECL development solution.
Arl13b protein has been shown to run at approximately 60-kDa. This is due to post-translational modifications (PMID:17488627).
Datasheets and documents
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SDS download
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Datasheet download
References (29)
ab136648 has been referenced in 29 publications.
- Sanchez GM et al. The β-cell primary cilium is an autonomous Ca2+ compartment for paracrine GABA signaling. J Cell Biol 222:N/A (2023). PubMed: 36350286
- Shankar S et al. Α γ-tubulin complex-dependent pathway suppresses ciliogenesis by promoting cilia disassembly. Cell Rep 41:111642 (2022). PubMed: 36384111
- Smit MJ et al. The developmental stage of the medulloblastoma cell-of-origin restricts Sonic hedgehog pathway usage and drug sensitivity. J Cell Sci 135:N/A (2022). PubMed: 35535520
- Hansen JN et al. A cAMP signalosome in primary cilia drives gene expression and kidney cyst formation. EMBO Rep 23:e54315 (2022). PubMed: 35695071
- Ijaz F et al. A pair of primers facing at the double-strand break site enables to detect NHEJ-mediated indel mutations at a 1-bp resolution. Sci Rep 12:11681 (2022). PubMed: 35804017