Recombinant Anti-ARL15 antibody [EPR12591] (ab178425)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12591] to ARL15
- Suitable for: WB, ICC/IF, IP
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-ARL15 antibody [EPR12591]
See all ARL15 primary antibodies -
Description
Rabbit monoclonal [EPR12591] to ARL15 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, IPmore details
Unsuitable for: Flow Cyt or IHC-P -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Recombinant fragment within Human ARL15. The exact sequence is proprietary.
Database link: Q9NXU5 -
Positive control
- K562, Human fetal kidney and fetal brain lysates; K562 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR12591 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab178425 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 23 kDa.
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ICC/IF |
1/50 - 1/100.
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IP |
1/10 - 1/100.
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Notes |
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WB
1/1000 - 1/10000. Predicted molecular weight: 23 kDa. |
ICC/IF
1/50 - 1/100. |
IP
1/10 - 1/100. |
Target
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Sequence similarities
Belongs to the small GTPase superfamily. Arf family. - Information by UniProt
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Database links
- Entrez Gene: 54622 Human
- Entrez Gene: 218639 Mouse
- Entrez Gene: 689079 Rat
- SwissProt: Q9NXU5 Human
- SwissProt: Q8BGR6 Mouse
- Unigene: 659125 Human
- Unigene: 79837 Mouse
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Alternative names
- A430036I03 antibody
- ADP ribosylation factor like 15 antibody
- ADP ribosylation factor like GTPase 15 antibody
see all
Images
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All lanes : Anti-ARL15 antibody [EPR12591] (ab178425) at 1/1000 dilution
Lane 1 : Human fetal kidney lysate
Lane 2 : K562 lysate
Lane 3 : Human fetal brain lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 23 kDa -
Immunofluorescent analysis of K562 cells labeling ARL15 with ab178425 at 1/50 dilution (red). DAPI nuclear staining (blue).
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Western blot analysis on immunoprecipitation pellet from (1) Human fetal kidney lysate or (2) 1X PBS (negative control) immunoprecipitated using ab178425 at 1/10 dilution. Detection utilized HRP-conjugated anti-rabbit IgG which preferentially detects the non-reduced form of rabbit IgG.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab178425 has not yet been referenced specifically in any publications.