Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12591] to ARL15
- Suitable for: WB, ICC/IF, IP
- Reacts with: Mouse, Rat, Human
Product nameAnti-ARL15 antibody [EPR12591]
See all ARL15 primary antibodies
DescriptionRabbit monoclonal [EPR12591] to ARL15
Tested applicationsSuitable for: WB, ICC/IF, IPmore details
Unsuitable for: Flow Cyt or IHC-P
Species reactivityReacts with: Mouse, Rat, Human
Recombinant fragment within Human ARL15. The exact sequence is proprietary.
Database link: Q9NXU5
- K562, Human fetal kidney and fetal brain lysates; K562 cells.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab178425 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 23 kDa.|
|ICC/IF||1/50 - 1/100.|
|IP||1/10 - 1/100.|
Sequence similaritiesBelongs to the small GTPase superfamily. Arf family.
- Information by UniProt
- A430036I03 antibody
- ADP ribosylation factor like 15 antibody
- ADP ribosylation factor like GTPase 15 antibody
All lanes : Anti-ARL15 antibody [EPR12591] (ab178425) at 1/1000 dilution
Lane 1 : Human fetal kidney lysate
Lane 2 : K562 lysate
Lane 3 : Human fetal brain lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 23 kDa
Immunofluorescent analysis of K562 cells labeling ARL15 with ab178425 at 1/50 dilution (red). DAPI nuclear staining (blue).
Western blot analysis on immunoprecipitation pellet from (1) Human fetal kidney lysate or (2) 1X PBS (negative control) immunoprecipitated using ab178425 at 1/10 dilution. Detection utilized HRP-conjugated anti-rabbit IgG which preferentially detects the non-reduced form of rabbit IgG.
ab178425 has not yet been referenced specifically in any publications.