Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6086] to ARL4A
- Suitable for: WB
- Reacts with: Human
Product nameAnti-ARL4A antibody [EPR6086]
See all ARL4A primary antibodies
DescriptionRabbit monoclonal [EPR6086] to ARL4A
Tested applicationsSuitable for: WBmore details
Unsuitable for: ICC/IF,IHC-P or IP
Species reactivityReacts with: Human
Does not react with: Mouse, Rat
Synthetic peptide within Human ARL4A aa 1-100. The exact sequence is proprietary.
- WB: Human small intestine lysate.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Dissociation constant (KD)KD = 9.34 x 10 -10 M Learn more about KD
Storage bufferpH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab124690 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 24 kDa (predicted molecular weight: 23 kDa).|
FunctionSmall GTP-binding protein which cycles between an inactive GDP-bound and an active GTP-bound form, and the rate of cycling is regulated by guanine nucleotide exchange factors (GEF) and GTPase-activating proteins (GAP). GTP-binding protein that does not act as an allosteric activator of the cholera toxin catalytic subunit. Recruits CYTH1, CYTH2, CYTH3 and CYTH4 to the plasma membrane in GDP-bound form.
Sequence similaritiesBelongs to the small GTPase superfamily. Arf family.
Cellular localizationCell membrane. Cytoplasm. Nucleus > nucleolus. Localization in the nucleolus is dependent by nucleotide binding.
- Information by UniProt
- ADP ribosylation factor like 4 antibody
- ADP ribosylation factor like 4A antibody
- ADP ribosylation factor like GTPase 4A antibody
Anti-ARL4A antibody [EPR6086] (ab124690) at 1/1000 dilution (Purified) + Human small intestine prepared in 1%SDS Hot lysis method at 15 µg
Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 23 kDa
Observed band size: 23 kDa
We recommend to use 1%SDS Hot lysis prepare method to get desired Western Blot results.
Blocking/Diluting Buffer and concentration: 5% NFDM/TBST
Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD
ab124690 has not yet been referenced specifically in any publications.