Recombinant Anti-ARL4A antibody [EPR6086] - BSA and Azide free (ab247961)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6086] to ARL4A - BSA and Azide free
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
Overview
-
Product name
Anti-ARL4A antibody [EPR6086] - BSA and Azide free
See all ARL4A primary antibodies -
Description
Rabbit monoclonal [EPR6086] to ARL4A - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Human
Does not react with: Mouse, Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
General notes
ab247961 is the carrier-free version of ab124690.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Dissociation constant (KD)
KD = 9.34 x 10 -10 M Learn more about KD -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
-
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR6086 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Conjugation kits
-
Isotype control
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab247961 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
Use at an assay dependent concentration. Detects a band of approximately 24 kDa (predicted molecular weight: 23 kDa).
|
Notes |
---|
WB
Use at an assay dependent concentration. Detects a band of approximately 24 kDa (predicted molecular weight: 23 kDa). |
Target
-
Function
Small GTP-binding protein which cycles between an inactive GDP-bound and an active GTP-bound form, and the rate of cycling is regulated by guanine nucleotide exchange factors (GEF) and GTPase-activating proteins (GAP). GTP-binding protein that does not act as an allosteric activator of the cholera toxin catalytic subunit. Recruits CYTH1, CYTH2, CYTH3 and CYTH4 to the plasma membrane in GDP-bound form. -
Sequence similarities
Belongs to the small GTPase superfamily. Arf family. -
Post-translational
modificationsMyristoylated. -
Cellular localization
Cell membrane. Cytoplasm. Nucleus > nucleolus. Localization in the nucleolus is dependent by nucleotide binding. - Information by UniProt
-
Database links
- Entrez Gene: 10124 Human
- Omim: 604786 Human
- SwissProt: P40617 Human
- Unigene: 245540 Human
-
Alternative names
- ADP ribosylation factor like 4 antibody
- ADP ribosylation factor like 4A antibody
- ADP ribosylation factor like GTPase 4A antibody
see all
Images
-
Anti-ARL4A antibody [EPR6086] (ab124690) at 1/1000 dilution (Purified) + Human small intestine tissue lysate prepared in 1% SDS Hot lysis method at 15 µg
Secondary
Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 23 kDa
Observed band size: 23 kDaThis data was developed using ab124690, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
We recommend to use 1%SDS Hot lysis prepare method to get desired Western Blot results.
-
This data was developed using ab124690, the same antibody clone in a different buffer formulation.Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
Datasheet download
Certificate of Compliance
References (0)
ab247961 has not yet been referenced specifically in any publications.