Overview

  • Product name

    Anti-ARL4A antibody - N-terminal
    See all ARL4A primary antibodies
  • Description

    Rabbit polyclonal to ARL4A - N-terminal
  • Host species

    Rabbit
  • Specificity

    ab199110 detects endogenous level of total ARL4A protein.

  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Fusion protein within Human ARL4A (N terminal). The exact sequence is proprietary. Recombinant fragment fusion protein corresponding to residues near the N terminal of human ARL4A. ANCBI Accession No. BC001111. The identity of the protein fusion partner is GST.
    Database link: P40617

  • Positive control

    • Human fetal liver tissue lysate and Human colon cancer tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab199110 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/200 - 1/1000. Predicted molecular weight: 23 kDa.

Target

  • Function

    Small GTP-binding protein which cycles between an inactive GDP-bound and an active GTP-bound form, and the rate of cycling is regulated by guanine nucleotide exchange factors (GEF) and GTPase-activating proteins (GAP). GTP-binding protein that does not act as an allosteric activator of the cholera toxin catalytic subunit. Recruits CYTH1, CYTH2, CYTH3 and CYTH4 to the plasma membrane in GDP-bound form.
  • Sequence similarities

    Belongs to the small GTPase superfamily. Arf family.
  • Post-translational
    modifications

    Myristoylated.
  • Cellular localization

    Cell membrane. Cytoplasm. Nucleus > nucleolus. Localization in the nucleolus is dependent by nucleotide binding.
  • Information by UniProt
  • Database links

  • Alternative names

    • ADP ribosylation factor like 4 antibody
    • ADP ribosylation factor like 4A antibody
    • ADP ribosylation factor like GTPase 4A antibody
    • ADP-ribosylation factor-like protein 4A antibody
    • ARL 4 antibody
    • ARL4 antibody
    • Arl4a antibody
    • ARL4A_HUMAN antibody
    see all

Images

  • Anti-ARL4A antibody - N-terminal (ab199110) at 1/533 dilution + Human fetal liver tissue lysate at 40 µg

    Predicted band size: 23 kDa

  • Immunohistochemical analysis of paraffin-embedded Human colon cancer tissue labeling ARL4A using ab199110 at a 1/50 dilution.

References

ab199110 has not yet been referenced specifically in any publications.

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