Key features and details
- Rabbit polyclonal to ARL4C
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-ARL4C antibody
DescriptionRabbit polyclonal to ARL4C
Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
Species reactivityReacts with: Human
antigen sequence corresponding to C terminal amino acids 88-192 of Human ARL4C.
- Human skeletal muscle tissue.
RT-4 and U-251 MG cell lysate.
- Human skeletal muscle tissue.
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab122025 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||Use a concentration of 0.04 - 0.4 µg/ml.|
|ICC/IF||Use a concentration of 0.25 - 2 µg/ml.|
FunctionSmall GTP-binding protein which cycles between an inactive GDP-bound and an active GTP-bound form, and the rate of cycling is regulated by guanine nucleotide exchange factors (GEF) and GTPase-activating proteins (GAP). GTP-binding protein that does not act as an allosteric activator of the cholera toxin catalytic subunit. May be involved in transport between a perinuclear compartment and the plasma membrane, apparently linked to the ABCA1-mediated cholesterol secretion pathway. Recruits CYTH1, CYTH2, CYTH3 and CYTH4 to the plasma membrane in the GDP-bound form. Regulates the microtubule-dependent intracellular vesicular transport from early endosome to recycling endosome process.
Tissue specificityExpressed in several tumor cell lines (at protein level). Expressed in lung, brain, leukocytes and placenta.
Sequence similaritiesBelongs to the small GTPase superfamily. Arf family.
Cellular localizationCell projection > filopodium. Cell membrane. Cytoplasm.
- Information by UniProt
- ADP ribosylation factor like 4C antibody
- ADP ribosylation factor like 7 antibody
- ADP-ribosylation factor-like protein 4C antibody
ab122025 at 1/120 dilution staining ARL4C in paraffin-embedded Human skeletal muscle by Immunohistochemistry.
All lanes : Anti-ARL4C antibody (ab122025) at 1/250 dilution
Lane 1 : RT-4 cell lysate.
Lane 2 : U-251 MG cell lysate.
Lane 3 : Human plasma
Lane 4 : Human Liver tissue lysate.
Lane 5 : Human Tonsil tissue lysate.
Developed using the ECL technique.
ab122025 at 1 µg/ml staining ARL4C (green) in PFA-fixed, Triton X-100 permeabilized, A-431 cells by Immunofluorescence.
ab122025 has not yet been referenced specifically in any publications.