Key features and details
- Rabbit polyclonal to ARL6IP1
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-ARL6IP1 antibody
DescriptionRabbit polyclonal to ARL6IP1
Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Human
Synthetic peptide corresponding to Human ARL6IP1 (C terminal).
Database link: Q15041
Previously labelled as ARMER.
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferPreservative: 0.02% Sodium azide
Concentration information loading...
Purification notesIon exchange chromatography purified IgG
Our Abpromise guarantee covers the use of ab24228 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 20 µg/ml.|
|WB||Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 27 kDa (predicted molecular weight: 23 kDa).|
|IHC-P||Use at an assay dependent concentration.|
FunctionMay be involved in protein transport, membrane trafficking, or cell signaling during hematopoietic maturation.
Tissue specificityExpressed in all hematopoietic cell lineages, but the highest level of expression is found in early myeloid progenitor cells. Expressed in brain, bone marrow, thymus and lung. Expressed at low level in liver, kidney and spleen. Not detected in heart.
Sequence similaritiesBelongs to the ARL6ip family.
Developmental stageDown-regulated during myeloid differentiation.
Cellular localizationEndomembrane system. Predominantly localized to intracytoplasmic membranes.
- Information by UniProt
- ADP ribosylation factor like 6 interacting protein antibody
- ADP-ribosylation factor-like protein 6-interacting protein 1 antibody
- Aip-1 antibody
Lane 1 : Anti-ARL6IP1 antibody (ab24228) at 0.5 µg/ml
Lane 2 : Anti-ARL6IP1 antibody (ab24228) at 1 µg/ml
Lane 3 : Anti-ARL6IP1 antibody (ab24228) at 2 µg/ml
All lanes : Human bladder cell lysate
Predicted band size: 23 kDa
Observed band size: 27 kDa why is the actual band size different from the predicted?
Western blot ana-lysis of ARL6IP1 in human bladder cell lysates with anti-ARL6IP1 at (A) 0.5, (B) 1, and (C) 2 µg/ml.
ab24228 at 2µg/ml staining of ARL6IP1 in human dladder tissue by IHC
ab24228 has been referenced in 1 publication.
- Huang HY et al. The embryonic expression patterns and the knockdown phenotypes of zebrafish ADP-ribosylation factor-like 6 interacting protein gene. Dev Dyn 238:232-40 (2009). PubMed: 19097054