Product nameAnti-ARL6IP2 antibody
See all ARL6IP2 primary antibodies
DescriptionRabbit polyclonal to ARL6IP2
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Horse, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Orangutan
Synthetic peptide within Human ARL6IP2 aa 400-450. The exact sequence is proprietary. (NP_001129145.1).
Database link: Q8NHH9
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab224825 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/1000. Predicted molecular weight: 66 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
FunctionGTPase tethering membranes through formation of trans-homooligomer and mediating homotypic fusion of endoplasmic reticulum membranes. Functions in endoplasmic reticulum tubular network biogenesis.
Tissue specificityExpressed in peripheral tissues (at protein level).
Sequence similaritiesBelongs to the GBP family. Atlastin subfamily.
Cellular localizationEndoplasmic reticulum membrane.
- Information by UniProt
- ADP ribosylation factor like 6 interacting protein 2 antibody
- ADP ribosylation factor like protein 6 interacting protein 2 antibody
- ADP-ribosylation factor-like protein 6-interacting protein 2 antibody
All lanes : Anti-ARL6IP2 antibody (ab224825) at 0.04 µg/ml
Lane 1 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg
Lane 2 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 15 µg
Lane 3 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Lane 4 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 66 kDa
Exposure time: 30 seconds
ARL6IP2 was immunoprecipitated from Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab224825 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab224825 at 0.4 µg/ml.
Lane 1: ab224825 IP in Jurkat whole cell lysate.
Lane 2: Control IgG IP in Jurkat whole cell lysate.
Detection: Chemiluminescence with exposure time of 10 seconds.
ab224825 has not yet been referenced specifically in any publications.