Anti-ARL6IP2 antibody (ab237631)
Key features and details
- Rabbit polyclonal to ARL6IP2
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-ARL6IP2 antibody
See all ARL6IP2 primary antibodies -
Description
Rabbit polyclonal to ARL6IP2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human ARL6IP2 aa 1-300.
Database link: Q8NHH9 -
Positive control
- WB: HepG2, MCF7, HL-60, K562, U-251 MG, A549 and A431 whole cell lysates. IHC-P: Human prostate cancer and colon cancer tissues. ICC/IF: HepG2 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Purity greater than 95%. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab237631 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/2000. Predicted molecular weight: 66 kDa.
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ICC/IF |
1/50 - 1/200.
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IHC-P |
1/20 - 1/200.
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Notes |
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WB
1/500 - 1/2000. Predicted molecular weight: 66 kDa. |
ICC/IF
1/50 - 1/200. |
IHC-P
1/20 - 1/200. |
Target
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Function
GTPase tethering membranes through formation of trans-homooligomer and mediating homotypic fusion of endoplasmic reticulum membranes. Functions in endoplasmic reticulum tubular network biogenesis. -
Tissue specificity
Expressed in peripheral tissues (at protein level). -
Sequence similarities
Belongs to the GBP family. Atlastin subfamily. -
Cellular localization
Endoplasmic reticulum membrane. - Information by UniProt
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Database links
- Entrez Gene: 64225 Human
- Omim: 609368 Human
- SwissProt: Q8NHH9 Human
- Unigene: 727652 Human
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Alternative names
- ADP ribosylation factor like 6 interacting protein 2 antibody
- ADP ribosylation factor like protein 6 interacting protein 2 antibody
- ADP-ribosylation factor-like protein 6-interacting protein 2 antibody
see all
Images
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Paraffin-embedded human prostate cancer tissue stained for ARL6IP2 using ab237631 at 1/100 dilution in immunohistochemical analysis.
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All lanes : Anti-ARL6IP2 antibody (ab237631) at 1/500 dilution
Lane 1 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 2 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate
Lane 3 : HL-60 (human promyelocytic leukemia cell line) whole cell lysate
Lane 4 : K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate
Lane 5 : U-251 MG (human brain glioma cell line) whole cell lysate
Lane 6 : A549 (human lung carcinoma cell line) whole cell lysate
Lane 7 : A431 (human epidermoid carcinoma cell line) whole cell lysate
Secondary
All lanes : Goat polyclonal to Rabbit IgG at 1/10000 dilution
Predicted band size: 66 kDa -
Paraffin-embedded human colon cancer tissue stained for ARL6IP2 using ab237631 at 1/100 dilution in immunohistochemical analysis.
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HepG2 (human liver hepatocellular carcinoma cell line) cells stained for ARL6IP2 (green) using ab237631 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488® conjugated Goat Anti-Rabbit IgG (H+L) secondary antibody.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab237631 has not yet been referenced specifically in any publications.