Key features and details
- Rabbit polyclonal to ARL6IP6
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-ARL6IP6 antibody
DescriptionRabbit polyclonal to ARL6IP6
Tested applicationsSuitable for: ICC/IF, IHC-P, WBmore details
Species reactivityReacts with: Human
- Human duodenum tissue; ARL6IP6 transfected HEK293T cell over-expression lysate
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab121448 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 0.25 - 2 µg/ml.|
|IHC-P||1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||Use a concentration of 0.04 - 0.4 µg/ml.|
Sequence similaritiesBelongs to the ARL6IP6 family.
- Information by UniProt
- ADP ribosylation like factor 6 interacting protein 6 antibody
- ADP-ribosylation factor-like protein 6-interacting protein 6 antibody
- Aip-6 antibody
All lanes : Anti-ARL6IP6 antibody (ab121448) at 1/250 dilution
Lane 1 : Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2 : Negative control (vector only transfected HEK293T lysate)
Lane 3 : ARL6IP6 transfected HEK293T over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa))
Developed using the ECL technique.
Immunofluorescent staining of Human cell line U-251MG shows positivity in nuclear membrane, cytoplasm and centrosome. Recommended concentration of ab121448 1-4 µg/ml. Cells treated with PFA/Triton X-100.
ab121448, at 1/225 dilution, staining ARL6IP6 in paraffin-embedded Human duodenum tissue by Immunohistochemistry.
ab121448 has not yet been referenced specifically in any publications.