Armenian Hamster IgG monoclonal [HTK888] - Isotype Control (ab18479)

Overview

  • Product name
    Armenian Hamster IgG monoclonal [HTK888] - Isotype Control
    See all Hamster isotype controls
  • Tested applications
    Suitable for: IHC-P, IHC-Fr, IP, WB, Flow Cytmore details
  • Immunogen

    Trinitrophenol and KLH

  • General notes
    Isotype control has been screened on a variety of resting, activated, live, and fixed mouse, rat and human tissues.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Monoclonal
  • Clone number
    HTK888
  • Isotype
    IgG
  • Research areas
  • Alternative names
    • Hamster Isotype Control

Applications

Our Abpromise guarantee covers the use of ab18479 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
IHC-Fr
IP
WB
Flow Cyt
  • Application notes
    Flow Cyt: Use at an assay dependent dilution for surface or intracellular staining.
    IHC-P: Use at an assay dependent dilution.
    IHC-Fr: Use at an assay dependent dilution.
    IP: Use at an assay dependent dilution.
    WB: Use at an assay dependent dilution. Predicted molecular weight: n/a kDa.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • References

    This product has been referenced in:
    • Hoffhines AJ  et al. Tyrosylprotein sulfotransferase-2 expression is required for sulfation of RNase 9 and Mfge8 in vivo. J Biol Chem 284:3096-105 (2009). Read more (PubMed: 19047058) »
    See 1 Publication for this product

    Customer reviews and Q&As

    1-5 of 5 Q&A

    Answer

    Thank you for your reply.

    I am sorry that you have been having issues with some of our antibodies in Flow Cytometry. Under our Abpromise we guarantee that ab108949, ab54427 & ab95780 should work in flow cytometry on rat samples. If we cannot resolve the issue you are having with the antibody then I would be happy to either send a replacement antibody or to process a refund.

    To be able to help you further in this matter, would you be able to send me a copy of the protocol that you have been using, along with any modifications that you have tried, to see if there is anything I can suggest.

    I look forward to your reply and helping resolve this matter.

    Read More

    Answer

    Thank you for your reply.

    I have sent the quote for the products requested to the email address we have on file for **********.

    When you are ready to convert the quote to an order, please contact our customer service team on 1-888-772-2226 or email to mailto:us.orders@abcam.com.

    If there is anything else I can help you with, please let me know.

    Read More

    Question
    Answer

    Thank you for your reply.

    Sorry to keep asking you questions, but the customer ID you gave me is for a Dr Alejandro Gomez, is that correct. I ask as its does not mentioned Bone Interface Group in their address that we have on file and I just wanted to make sure that the quote went to the correct person.

    Thank you for your patience, and I look forward to your reply.

    Read More

    Answer

    Thank you for contacting us with your question. I contacted the production lab to enquire about the isotype of ab36436, and unfortunately they said that the antibody has not been characterized to distinguish if it is IgG1 or IgG2.   We do have hamster IgG isotype controls that you may use as control antibodies. I would recommend ab18479, which is an Armenian Hamster IgG isotype control. This antibody has been tested on a variety of mouse, rat, and human tissues. I hope this information is helpful, but please let me know if you have further questions.  

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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