Armenian Hamster IgG monoclonal [HTK888] - Isotype Control (ab18479)

Q&A (5)References (2)

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Overview

  • Product name

    Armenian Hamster IgG monoclonal [HTK888] - Isotype Control
    See all Hamster isotype controls

  • Tested applications

    Suitable for: IHC-P, IHC-Fr, IP, WB, Flow Cytmore details

  • Immunogen

    Chemical/ Small Molecule corresponding to Hamster. Trinitrophenol and KLH

  • General notes

    Isotype control has been screened on a variety of resting, activated, live, and fixed mouse, rat and human tissues.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C.

  • Storage buffer

    pH: 7.20
    Preservative: 0.09% Sodium azide
    Constituent: PBS
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Clonality

    Monoclonal

  • Clone number

    HTK888

  • Isotype

    IgG

  • Research areas

  • Alternative names

    • Hamster Isotype Control

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab18479 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
IHC-Fr
IP
WB
Flow Cyt
Notes
Application notes
Flow Cyt: Use at an assay dependent dilution for surface or intracellular staining.
IHC-P: Use at an assay dependent dilution.
IHC-Fr: Use at an assay dependent dilution.
IP: Use at an assay dependent dilution.
WB: Use at an assay dependent dilution. Predicted molecular weight: n/a kDa.

Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

References (2)

Publishing research using ab18479? Please let us know so that we can cite the reference in this datasheet.

ab18479 has been referenced in 2 publications.

  • Matsuo K  et al. Innervation of the tibial epiphysis through the intercondylar foramen. Bone 120:297-304 (2018). PubMed: 30439572
  • Hoffhines AJ  et al. Tyrosylprotein sulfotransferase-2 expression is required for sulfation of RNase 9 and Mfge8 in vivo. J Biol Chem 284:3096-105 (2009). PubMed: 19047058

Customer reviews and Q&As

Q&A
Submit a question

1-5 of 5 Q&A

Question

We have purchased some antibodies against rat platelets:

Primary:

ab108949 - mouse monoclonal anti CD63, FITC labeled
(ab106163 as isotype control)

ab54427 - mouse monoclonal anti CD62P
(ab91353 as the isotype control, as a secondary antibody)

ab95780 anti CD42d armenian hamster antibody
(ab18479 as an isotype control, for which we are using APC-conjugated Goat Anti-Hamster IgG from Leinco).

We are ibodies.doing flow cytometry experiments on fixed whole blood, and fixed platelet-rich plasma. The problem is that the samples with the isotype control show the same fluorescence as the samples with the primary ant We have tried a number of different fixing and staining protocols, with and without washing, but until now had no success. What I am wondering about is, how reliable are these primary antibodies for the use with rat platelets?

Some help would be greatly appreciated.

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Answer

Thank you for your reply.

I am sorry that you have been having issues with some of our antibodies in Flow Cytometry. Under our Abpromise we guarantee that ab108949, ab54427 & ab95780 should work in flow cytometry on rat samples. If we cannot resolve the issue you are having with the antibody then I would be happy to either send a replacement antibody or to process a refund.

To be able to help you further in this matter, would you be able to send me a copy of the protocol that you have been using, along with any modifications that you have tried, to see if there is anything I can suggest.

I look forward to your reply and helping resolve this matter.

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Question

Yes, it is correct. ************does the purchasing in this group usually. I am a visiting professor here.

Feel free to contact him to double-check, he is the one who gave me the customer ID.

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Answer

Thank you for your reply.

I have sent the quote for the products requested to the email address we have on file for **********.

When you are ready to convert the quote to an order, please contact our customer service team on 1-888-772-2226 or email to mailto:us.orders@abcam.com.

If there is anything else I can help you with, please let me know.

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Question

Concerning the quote, the customer number is**********

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Answer

Thank you for your reply.

Sorry to keep asking you questions, but the customer ID you gave me is for a Dr Alejandro Gomez, is that correct. I ask as its does not mentioned Bone Interface Group in their address that we have on file and I just wanted to make sure that the quote went to the correct person.

Thank you for your patience, and I look forward to your reply.

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Question

I selected the following antibodies from the ABcam catalog:

Primary:

Anti-CD63 antibody [AD1] (FITC)

ab108949

$337.00



Anti-CD62P antibody [Psel.KO.2.7]

ab54427

$337.00



Anti-CD42d antibody [1C2]

ab95780

$337.00



Secondary:


Goat anti-Mouse IgG H&L (Cy5.5 ®)

ab6947

$185.00



Goat anti-Mouse IgG1 heavy chain (FITC)

ab97239

$165.00



Goat polyclonal to Armenian Hamster (TR)

ab5743

$165.00



Isotype controls:


MOUSE IgG1 antibody [MOPC-21] (FITC)

ab106163

$325.00



Mouse IgG1 [ICIGG1] - Isotype Control

ab91353

$315.00



Armenian Hamster IgG monoclonal [HTK888] - Isotype control

ab18479

$325.00



Two questions:

For the secondary antibody, will the first one (ab6947) work against ab54427, or do we need to buy the second one (ab97239)? One is against IgG, the other one is against IgG1. ab54427 is IgG1.

If you could double-check the selection for compatibility (e.g., correct isotype controls) and then ask the sales department to offer us a quote, that would be great.

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Answer

Thank you for your reply.

The antibodies you selected look good to me. To generate a quote we do need to have a shipping address and in your last email, you mentioned that you were at the University of Toronto at the moment. Could you please send me your current email address and I will generate the quote.

I look forward to your reply.

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Question

I need to use a control antibody for a functional study with your Integrin beta 3 antibody(Cat. ab36436). Could you please let me know which form of Ig is it ? for example: Hamster IgG (IgG1 or G2)?

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Answer

Thank you for contacting us with your question. I contacted the production lab to enquire about the isotype of ab36436, and unfortunately they said that the antibody has not been characterized to distinguish if it is IgG1 or IgG2.   We do have hamster IgG isotype controls that you may use as control antibodies. I would recommend ab18479, which is an Armenian Hamster IgG isotype control. This antibody has been tested on a variety of mouse, rat, and human tissues. I hope this information is helpful, but please let me know if you have further questions.  

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