Overview

  • Product name
  • Description
    Rabbit polyclonal to Aromatase
  • Host species
    Rabbit
  • Specificity
    ab18995 recognises aromatase.
  • Tested applications
    Suitable for: ICC/IF, IHC-Fr, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Chicken, Cow, Pig
  • Immunogen

    Synthetic peptide surrounding amino acid 385 of human Aromatase (Peptide available as ab51924.)

  • Positive control
    • ICC/IF: HepG2 cells. IHC-P: Human normal placenta.

Properties

Applications

Our Abpromise guarantee covers the use of ab18995 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 µg/ml.
IHC-Fr Use at an assay dependent concentration. PubMed: 19620711
IHC-P Use a concentration of 10 - 20 µg/ml.
WB Use a concentration of 0.5 - 4 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 58 kDa).Can be blocked with Aromatase peptide (ab51924). An additional 35 kDa band can also be detected in Jurkat cells (we are unsure of the identity).

Target

  • Function
    Catalyzes the formation of aromatic C18 estrogens from C19 androgens.
  • Tissue specificity
    Brain, placenta and gonads.
  • Involvement in disease
    Defects in CYP19A1 are a cause of aromatase excess syndrome (AEXS) [MIM:139300]; also known as familial gynecomastia. AEXS is characterized by an estrogen excess due to an increased aromatase activity.
    Defects in CYP19A1 are the cause of aromatase deficiency (AROD) [MIM:107910]. AROD is a rare disease in which fetal androgens are not converted into estrogens due to placental aromatase deficiency. Thus, pregnant women exhibit a hirsutism, which spontaneously resolves after post-partum. At birth, female babies present with pseudohermaphroditism due to virilization of extern genital organs. In adult females, manifestations include delay of puberty, breast hypoplasia and primary amenorrhoea with multicystic ovaries.
  • Sequence similarities
    Belongs to the cytochrome P450 family.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • ARO antibody
    • ARO1 antibody
    • Aromatase antibody
    • CP19A_HUMAN antibody
    • CPV1 antibody
    • CYAR antibody
    • CYP19 antibody
    • Cyp19a1 antibody
    • CYPXIX antibody
    • Cytochrome P-450AROM antibody
    • Cytochrome P450 19A1 antibody
    • Cytochrome P450, family 19, subfamily A, polypeptide 1 antibody
    • Cytochrome P450, subfamily XIX (aromatization of androgens) antibody
    • Estrogen synthase antibody
    • Estrogen synthetase antibody
    • Flavoprotein linked monooxygenase antibody
    • MGC104309 antibody
    • Microsomal monooxygenase antibody
    • OTTHUMP00000162543 antibody
    • OTTHUMP00000198350 antibody
    • P 450AROM antibody
    see all

Images

  • Ab18995 staining Human normal placenta. Staining is localised to cell membrane.
    Left panel: with primary antibody at 4 ug/ml. Right panel: isotype control.
    Sections were stained using an automated system DAKO Autostainer Plus , at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer citrate pH6.1 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
  • ICC/IF image of ab18995 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab18995, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Anti-Aromatase antibody (ab18995) at 1 µg/ml + Human brain normal tissue lysate - membrane extract (ab29456) at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 58 kDa
    Observed band size: 58 kDa
    Additional bands at: 34 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 1 minute

References

This product has been referenced in:
  • Yan L  et al. Effects of Ovariectomy in an hSOD1-G93A Transgenic Mouse Model of Amyotrophic Lateral Sclerosis (ALS). Med Sci Monit 24:678-686 (2018). Read more (PubMed: 29394243) »
  • Zhang L  et al. A GABAergic cell type in the lateral habenula links hypothalamic homeostatic and midbrain motivation circuits with sex steroid signaling. Transl Psychiatry 8:50 (2018). Rat . Read more (PubMed: 29479060) »
See all 28 Publications for this product

Customer reviews and Q&As

1-10 of 24 Abreviews or Q&A

Answer

Thank you for getting back to me.

I have seen the correspondence you have had with my colleague Claudia over the ab4360 slide, she wrote the following:

These slides do not automatically contain this region (syncytiotrophoblast area), we can however might provide this on a custom order for you, depending on the syncytiotrophoblast area you are interested in. Are you interested in the baby side or the uterus side? If you would like the tissue from the baby side, we are able to do it, otherwise, unfortunately not.

Please let me know whether you are interested in such slides and we can make you a proposal for these custom made slides for you.

Would you be interested in this?

The testing discount I mentioned in my previous emailin relation toab113236 is like a two for one offer. You would initially purchase ab113236 full price (or in this case, with a 5% discount), then test it with your rat samples and let us know of the results in an Abreview. You would then be entitled to a free primary antibody of your choice from our catalogue. More information on how this offer works can be found from the following link:

https://www.abcam.com/collaboratordiscount

If you would like me to put together a quote could you please confirm if you are still interested in the ab4360 slide? Also, couldyou please confirm if you would be interestedin participating tin the testing discount scheme and I will generate the discount code for you.

I look forward to recieving your reply.

Read More

Answer

Thank you for contacting us and your interest in our products. In answer to your questions:

1. Yes, rabbit polyclonal IgG isotype ab27478 would be an appropriate isotype control for the experiment you are hoping to perform with anti- Cytochrome P450 26B (ab113236). What I would say however is that ab 113236 has as yet not been tested with rat samples to our knowledge. This does not meant that it would not detect the rat epitope, however we cannot guarantee that it would as we have not tested it. The immunogen used to raise this antibody is the recombinantly expressed Human Cytochrome P450 26B. This sequence shares 96 % homology with the rat protein and would therefore be predicted to react (as stated on the datasheet of this antibody). If you would be interested, I would be able to offer you a testing discount for this antibody (please see below for more information on this offer).

2.. Ab126785 - primary antibody for anti-cyp27A1. Should I use Ab27478 or Ab27472? Either isotype control should be fine, the main difference between the two products is that ab27472 is prediluted at a concentration of 2 µg/ml so it may be better to buyab27478 as this can be diluted to higher concentrations if needed. It may be a problem that the concentration of ab126785 is not stated, I am contacting the source of this antibody to find out the concentration so that you can work outthe appropriate level of isotype control to use. I will get back to you with this information, it may however be that it has not been assessed as the product is supplied in the form of a culture supernatant.

3. Ab18995 - primary antibody for anti-aromatase. Ab27478 would be fine to use as an isotype control for this antibody. Even though ab113263 and ab18995 are polyclonalantibodies, I think using an isotype control will be a useful test to assessany non-specificstainingyou may encounter.

May I ask, have youchosen any secondary antibody for the stainingexperiments? We have several antibodies which may be appropriate for you.These can be foundby specifyingyour conjugate preference, and any other details important to you through the following search engine:

https://www.abcam.com/index.html?pageconfig=productmap&cl=918

Or if yougive me a few details ofthe preferences you have I may be able to suggest somesuitable secondary antibodies from our catalogue.

As mentioned above. If you would be interested, you would be eligible for a testing discount if you would like to purchase ab113263. As thisantibody has not been used with rat samples previously, if you were to purchase this antibody, test it with your rat samples, and share the results with us through an Abreview (should only take 5-10 minutes), then you would be eligible for a free primary antibody of your choice from our catalogue. You would be entitled to this free primary antibody whether the result are positive or negative, we just want to reward you for providing us with the information. The only constraint is that ab113263 needs to be tested, the review submitted and the free antibody claimed within 4 months. More information on this scheme can be found from the following link:

https://www.abcam.com/collaboratordiscount

If you would like to participate in this scheme please do let me know as a discount code needs to be issued prior to purchasing of the antibody.

As you are intending to purchase more than 5 items in one order I am also able to offer you a 5% discounton the items in your order.

I hope this information has been of help. If you requireany further informationplease do not hesitate to contact usagain.

Read More
Application
Immunohistochemistry (Frozen sections)
Sample
Pig Tissue sections (Ovary)
Permeabilization
No
Specification
Ovary
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Fixative
Paraformaldehyde

Natasja Costermans

Verified customer

Submitted Aug 09 2018

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Rat Placenta)
Antigen retrieval step
Enzymatic
Permeabilization
No
Specification
Rat Placenta
Blocking step
Serum as blocking agent for 4 hour(s) and 0 minute(s) · Concentration: 1.5%
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Mar 29 2016

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Mouse Tissue sections (Spinal cord)
Antigen retrieval step
None
Permeabilization
Yes - TritonX 100
Specification
Spinal cord
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Sep 29 2015

Answer



I have had a look at the western blot from the other distributor and it does indeed show two bands at similar molecular weights to the ones shown on our datasheet. We have also looked at similar antibodies from other companies and their antibodies also show these two characteristic bands at ˜34kDA and ˜58 kDa.

Some of these other suppliers make reference to the lower band possibly being the cleavage product of Aromatase however, we have not yet been able to find any online data regarding a potential cleavage product and therefore there is no data to support this hypothesis.

Since our last correspondence, we have also found http://humrep.oxfordjournals.org/content/24/9/2133.full which indicates that the lower band is not specific (by sequencing).

Hence, at present, we are still unsure as to the identity of this lower band.

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (pancreatic tumor)
Specification
pancreatic tumor
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate
Permeabilization
No
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Abcam user community

Verified customer

Submitted Oct 08 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (pancreatic tumor)
Specification
pancreatic tumor
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate
Permeabilization
No
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Abcam user community

Verified customer

Submitted Oct 05 2012

Answer

Thank you very much for your interest in ab18995.

To our knowledge, ab18995 has not been tested in fish. We do not carry any sample sizes due to our extremely large catalog of products. We do however have a testing discount program. Therefore, I can offer a discount off a future purchase if you buy ab18995 now, test it in fish and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of: 1 free PRIMARY ANTIBODY..

If you are interested in this offer, please follow these steps:

1. Reply to this e-mail to let me know that you would like to proceed and test ab18995 in fish. I will then send a discount code. This code must be issued before purchasing ab18995 so please wait for my reply before ordering.

2. Purchase ab18995 either by phone, fax, or online (www.abcam.com).

3. Test it in fish.

4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any PRIMARY ANTIBODY ordered and the discount code is valid for 4 months after issue.
Please remember that submission of the Abreview is sufficient for the discount code to become active.

We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab18995 turns out to be unsuitable for fish, you will still receive the discount on your next purchase after your Abreview has been submitted.

Please let me know if you have any questions about this offer and I would be happy to help you further.

The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

Read More

Answer

Thank you your reply.

Your credit note ID is **.

I am sorry that this antibody did not perform as stated on the datasheet. If payment has already been made on the original order and you wish to receive a refund, please ask your purchasing department to contact our accounting department so that we may gather the correct information needed for the refund. To avoid confusion, please ensure your accounts department is aware of how the credit note is being used.

Our accounting department can be contacted by email at us.credits@abcam.com or by telephone using the information at the Contact Us link in the top right corner of our website. Please refer to the credit note ID in any correspondence with our accounting department.

The credit note ID is for your reference only and does not automatically guarantee the credit.

I hope this experience will not prevent you from purchasing other products from us in the future. Our Scientific Support team is always at your service, should you require further expert advice.

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1-10 of 24 Abreviews or Q&A

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