Product nameAnti-Aromatase antibody
See all Aromatase primary antibodies
DescriptionRabbit polyclonal to Aromatase
Tested applicationsSuitable for: WB, IHC-Frmore details
Species reactivityReacts with: Dog, Human
Predicted to work with: Rabbit
- Recombinant Human Aromatase protein (ab114260) can be used as a positive control in WB. This antibody gave a positive signal in Human placenta tissue lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
PurityImmunogen affinity purified
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipid metabolism
Immunizing Peptide (Blocking)
Our Abpromise guarantee covers the use of ab35604 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 58 kDa).|
FunctionCatalyzes the formation of aromatic C18 estrogens from C19 androgens.
Tissue specificityBrain, placenta and gonads.
Involvement in diseaseDefects in CYP19A1 are a cause of aromatase excess syndrome (AEXS) [MIM:139300]; also known as familial gynecomastia. AEXS is characterized by an estrogen excess due to an increased aromatase activity.
Defects in CYP19A1 are the cause of aromatase deficiency (AROD) [MIM:107910]. AROD is a rare disease in which fetal androgens are not converted into estrogens due to placental aromatase deficiency. Thus, pregnant women exhibit a hirsutism, which spontaneously resolves after post-partum. At birth, female babies present with pseudohermaphroditism due to virilization of extern genital organs. In adult females, manifestations include delay of puberty, breast hypoplasia and primary amenorrhoea with multicystic ovaries.
Sequence similaritiesBelongs to the cytochrome P450 family.
- Information by UniProt
- ARO antibody
- ARO1 antibody
- Aromatase antibody
Anti-Aromatase antibody (ab35604) at 1 µg/ml + Human placenta tissue lysate - total protein (ab29745) at 20 µg
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 58 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted?
Exposure time: 150 seconds
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab35604 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
ab35604 staining Aromatase in dog ovary tissue section by Immunohistochemistry (Frozen sections). Tissue underwent fixation in acetone for 10 minutes and blocked in TBS buffer (1%BSA + 10%NGS) for 2 hours at 25°C. The primary antibody was diluted 1/50 and incubated with sample for 16 hours at 4°C. A HRP conjugated goat polyclonal to rabbit IgG (H&L), diluted 1/100 was used as secondary.
This product has been referenced in:
- Zarate-Perez F et al. Biophysical characterization of Aptenodytes forsteri cytochrome P450 aromatase. J Inorg Biochem 184:79-87 (2018). WB . Read more (PubMed: 29684698) »
- Adurthi S et al. Oestrogen Receptor-a binds the FOXP3 promoter and modulates regulatory T-cell function in human cervical cancer. Sci Rep 7:17289 (2017). IHC-P . Read more (PubMed: 29229929) »