Overview

Description

  • Nature
    Synthetic

Associated products

Specifications

Our Abpromise guarantee covers the use of ab51924 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Blocking - Blocking peptide for Anti-Aromatase antibody (ab18995)

  • Purity
    > 90 % SDS-PAGE.

  • Form
    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    Preservative: 0.02% Thimerosal (merthiolate)
    Constituents: 0.1% BSA, PBS, pH 7.2

General Info

  • Alternative names
    • ARO
    • ARO1
    • Aromatase
    • CP19A_HUMAN
    • CPV1
    • CYAR
    • CYP19
    • Cyp19a1
    • CYPXIX
    • Cytochrome P-450AROM
    • Cytochrome P450 19A1
    • Cytochrome P450, family 19, subfamily A, polypeptide 1
    • Cytochrome P450, subfamily XIX (aromatization of androgens)
    • Estrogen synthase
    • Estrogen synthetase
    • Flavoprotein linked monooxygenase
    • MGC104309
    • Microsomal monooxygenase
    • OTTHUMP00000162543
    • OTTHUMP00000198350
    • P 450AROM
    see all
  • Function
    Catalyzes the formation of aromatic C18 estrogens from C19 androgens.
  • Tissue specificity
    Brain, placenta and gonads.
  • Involvement in disease
    Defects in CYP19A1 are a cause of aromatase excess syndrome (AEXS) [MIM:139300]; also known as familial gynecomastia. AEXS is characterized by an estrogen excess due to an increased aromatase activity.
    Defects in CYP19A1 are the cause of aromatase deficiency (AROD) [MIM:107910]. AROD is a rare disease in which fetal androgens are not converted into estrogens due to placental aromatase deficiency. Thus, pregnant women exhibit a hirsutism, which spontaneously resolves after post-partum. At birth, female babies present with pseudohermaphroditism due to virilization of extern genital organs. In adult females, manifestations include delay of puberty, breast hypoplasia and primary amenorrhoea with multicystic ovaries.
  • Sequence similarities
    Belongs to the cytochrome P450 family.
  • Cellular localization
    Membrane.
  • Information by UniProt

References

This product has been referenced in:
  • Charif SE  et al. Local production of neurostradiol affects gonadotropin-releasing hormone (GnRH) secretion at mid-gestation inLagostomus maximus(Rodentia, Caviomorpha). Physiol Rep 5:N/A (2017). Read more (PubMed: 29038356) »
See 1 Publication for this product

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

Answer

Thank you for your message and for providing this further information.

I am sorry to hear suggestions and use of the blocking peptide have not improved the results on this occasion. It does appear as this vial of ab18995 is bad. I appreciate the time you have spent on these experiments, and if this product has been purchased within the last 6 months, would like to offer a replacement, refund or credit note in compensation. In order to arrange this, I would appreciate if you could confirm your order number and date of purchase?

Thank you for your continued cooperation. I look forward to hearing from you with details of how you would like to proceed.

Read More

Answer

Thank you for contacting us. Your credit note IDs are I am sorry that these products did not perform as stated on the datasheet, I have asked our Finance department to issue a credit note for you. The credit note may be used in one of the following ways: (1) Redeemed against the original invoice if this hasn't already been paid. (2) Held on the account for use against a future order. (3) A full refund can be offered where no other invoices are outstanding. Please contact your Finance department to confirm how you would like the credit note to be used and ensure it is not redeemed without your knowledge. To specifically receive a refund please ask your Finance department to contact our Finance department at creditcontrol@abcam.com or by telephone using the information at the “Contact Us” link in the top right corner of our website. The credit note ID is for your reference only, please refer to the credit note ID in any correspondence with our accounting department. We will send you the completed credit note by email or postal mail with the actual credit note number which will start with the letters CGB. I hope this experience will not prevent you from purchasing other products from us in the future. Our Scientific Support team is always at your service should you require further expert advice

Read More

Answer

Thank you for contacting us. We believe that the best practices are to give the customer as much data as possible about any product. Therefore, all data that we possess and may share publically concerning our products is listed on our website. This blocking peptide ab51924 is actually the immunogen of the anitibody ab18995.By its nature the antibody should block to the immunogen preferentially. I am unsure why the customer in the Abreview was unable to use this effectively but would think that it may be that not enough peptide was used or that the incubation period was too short. If you wish to know more about this, I have included our blocking peptide protcol. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Read More
Application
Western blot

Review text: How long did you incubate the protein and antibody mix before adding it to the blot?
First Time: Incubation time of antibody and protein (peptide): two hour at room temperature
Second Time:Incubation time of antibody and protein (peptide): One hour at 37 degree C
How much antibody/protein mix was added to the blot?
Antibody used for the reaction is 1ug and protein (peptide) 5ug (1:5 ratio)
Sample: Mouse Tissue lysate - other (BRAIN)
Loading amount: 15 µg
Specification: BRAIN
Blocking step: Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details
Concentration: 5 µg/ml
Incubation time: 1 hour(s) and 0 minute(s) · Temperature: 30°C

Primary antibody (in addition to 'Aromatase peptide')
Concentration: 1 µg/ml

Secondary antibody
Name: Non-Abcam antibody was used: anti - rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution: 1/20000

Detection
Detection method: ECL
Exposure: 2 minute(s) and 0 second(s)
Bands: Specific: 54 kDa Non-specific: 104, 77, 71, 67, 46, 36, kDa

Additional data
Additional Notes: AROMATASE PEPTIDE DON'T BLOCK ANY BANDS.
Peptide usage 5 times - 1ug of antibody and 5ug of blocking peptide.
Abcam response: The blocking peptide was the same as the immunogen. At the moment we have not found a reason why blocking is not taking place,
We have not had similar complaints for Aromatase peptide (ab51924).
More blocking peptide might be needed in customer's case.

Abcam user community

Verified customer

Submitted Apr 28 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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