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Hi, My name is Laura and I'm working with your aromatase ab18995 antibody. In my blots, I observe several bands other than 36 KDa and 58 KDa after incubation with this antibody in 5% milk/TBS-T0.2% at a dilution of 1/1000. On your website I found an antigen (ab 51924) that eliminates non-specific bands. However, I read testimonies of other researchers on your website that tried this products with a little or no sucess. Before I try this antigen I want to be sure that your product blocks the non specific signals. I would like to ask you if you could provide me with some blots that demostrate this effect, and if it is possible to know the experimental conditions that have been performed. Best regards, Laura Lugo PhD. Thomas Jefferson University phone#: 215 503 5565
Asked on Jan 12 2012
Thank you for contacting us. We believe that the best practices are to give the customer as much data as possible about any product. Therefore, all data that we possess and may share publically concerning our products is listed on our website. This blocking peptide ab51924 is actually the immunogen of the anitibody ab18995.By its nature the antibody should block to the immunogen preferentially. I am unsure why the customer in the Abreview was unable to use this effectively but would think that it may be that not enough peptide was used or that the incubation period was too short. If you wish to know more about this, I have included our blocking peptide protcol. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.
Answered on Jan 12 2012