Product nameAnti-ARP1 antibody
See all ARP1 primary antibodies
DescriptionRabbit polyclonal to ARP1
Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: Dog
General notesIf slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.
Previously labelled as ACTR1A.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.097% Sodium azide
Constituent: 0.0268% PBS
Concentration information loading...
Purification notesWhole antiserum is fractionated and then further purified by ion-exchange chromatography to provide the IgG fraction of antiserum that is essentially free of other rabbit serum proteins.
Our Abpromise guarantee covers the use of ab11009 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
For indirect immunofluorescence, a minimum working antibody dilution of 1:100 is recommended using mouse fibroblasts NIH3T cell.
WB: 1/1000 (recommended using a rat brain cytosol extract and a whole cell extract of the human epidermoid carcinoma A431 cell line). Predicted molecular weight: 45 kDa.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionComponent of a multi-subunit complex involved in microtubule based vesicle motility. It is associated with the centrosome.
Sequence similaritiesBelongs to the actin family. ARP1 subfamily.
Cellular localizationCytoplasm > cytoskeleton. Cytoplasm > cytoskeleton > centrosome.
- Information by UniProt
- Actin related protein 1 antibody
- Actin RPV antibody
- Actin-RPV antibody
Immunohistochemistry (Formalin fixed, paraffin-embedded sections) analysis of rat duodenum tissue labelling ARP1 with ab11009.
Immunohistochemistry (Formalin fixed, paraffin-embedded sections) analysis of rat kidney tissue labelling ARP1 with ab11009.
All lanes : Anti-ARP1 antibody (ab11009) at 1/1000 dilution
Lane 1 : A432 cell lysate
Lane 2 : A432 cell lysate with ARP1 immunising peptide at 5 mg/ml
Predicted band size: 45 kDa
Observed band size: 45 kDa
This product has been referenced in:
- Dwivedi D et al. The dynein adaptor Hook2 plays essential roles in mitotic progression and cytokinesis. J Cell Biol 218:871-894 (2019). Read more (PubMed: 30674580) »
- Kamal AHM et al. Cross-linking Proteomics Indicates Effects of Simvastatin on the TLR2 Interactome and Reveals ACTR1A as a Novel Regulator of the TLR2 Signal Cascade. Mol Cell Proteomics 18:1732-1744 (2019). Read more (PubMed: 31221720) »