Anti-A3H antibody (ab73055)
Key features and details
- Mouse polyclonal to A3H
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-A3H antibody -
Description
Mouse polyclonal to A3H -
Host species
Mouse -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein within Human A3H aa 1-200. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
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Positive control
- Human colon tissue lysate and transfected 293 cell line lysate
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.4
Constituents: Whole serum, 0.812% Sodium chloride, 0.0225% Potassium chloride, 0.0204% Monobasic dihydrogen potassium phosphate, 0.1136% Dibasic monohydrogen sodium phosphate -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab73055 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/1000. Detects a band of approximately 19,20 kDa.
Expected band of 21.5 kDa (Isoform 2). |
Notes |
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WB
1/500 - 1/1000. Detects a band of approximately 19,20 kDa. Expected band of 21.5 kDa (Isoform 2). |
Target
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Relevance
ARP10 belongs to the cytidine and deoxycytidylate deaminase family. It is a poorly expressed deaminase that is ineffective at inhibiting retroviral replication. ARP10 has DNA deaminase (cytidine deaminase) activity but is not able to mediate G-to-A hypermutation in newly synthesized retroviral DNA. It is present at low level in 293T cells. It is considered an unstable protein, suggesting that it may be degraded by the proteasome (at protein level). Weakly expressed in testis, ovary, fetal liver and skin. There are two named isoforms. ARP10 from old world monkeys has retained its antiviral activity, while it is lost in other primates. It is one of seven related genes or pseudogenes found in a cluster, thought to result from gene duplication, on chromosome 22. -
Cellular localization
Nucleus. Cytoplasm. Note: Mostly nuclear. -
Database links
- Entrez Gene: 164668 Human
- Omim: 610976 Human
- SwissProt: Q6NTF7 Human
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Alternative names
- A3H antibody
- APOBEC related protein 10 antibody
- APOBEC3H antibody
see all
Images
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Anti-A3H antibody (ab73055) at 1/500 dilution + human colon tissue lysate at 25 µg
Secondary
Goat anti-mouse IgG (H&L)-HRP conjugate at 1/2500 dilution
Observed band size: 20 kDa why is the actual band size different from the predicted? -
All lanes : Anti-A3H antibody (ab73055) at 1/500 dilution
Lane 1 : A3H transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat anti-mouse IgG (H&L)-HRP conjugate at 1/2500 dilution
Observed band size: 19 kDa why is the actual band size different from the predicted?
Datasheets and documents
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Datasheet download
References (1)
ab73055 has been referenced in 1 publication.
- Desimmie BA et al. APOBEC3 proteins can copackage and comutate HIV-1 genomes. Nucleic Acids Res 44:7848-65 (2016). PubMed: 27439715