Overview

  • Product name

  • Description

    Rabbit polyclonal to Arp2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, IHC-Pmore details
    Unsuitable for: WB
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Chicken, Cow, Orangutan
  • Immunogen

    Synthetic peptide within Human Arp2 aa 250-300. The exact sequence is proprietary.
    Database link: P61160

  • Positive control

    • IP: HEK-293T whole cell lysate. IHC-P: Human skin squamous cell carcinoma.

Properties

Applications

Our Abpromise guarantee covers the use of ab226467 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 2-10 µg/mg of lysate.
IHC-P 1/1000 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for WB.
  • Target

    Images

    • Arp2 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab226467 at 6 µg per reaction. Western blot was performed from the immunoprecipitate using ab226467 at 1 µg/ml.

      Lane 1: ab226467 IP in HEK-293T whole cell lysate.

      Lane 2: Control IgG IP in HEK-293T whole cell lysate.

      Detection: Chemiluminescence with exposure time of 10 seconds.

      Cells prepared using NETN lysis buffer.

    • Formalin-fixed, paraffin-embedded human skin squamous cell carcinoma tissue stained for Arp2 using ab226467 at 1/5000 dilution in immunohistochemical analysis.

      Detection: DAB staining. Counterstain: Hematoxylin (blue).

    References

    ab226467 has not yet been referenced specifically in any publications.

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