Key features and details
- Rabbit polyclonal to Arp2
- Suitable for: IP, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Arp2 antibody
See all Arp2 primary antibodies
DescriptionRabbit polyclonal to Arp2
Tested applicationsSuitable for: IP, IHC-Pmore details
Unsuitable for: WB
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Chicken, Cow, Orangutan
Synthetic peptide within Human Arp2 aa 250-300. The exact sequence is proprietary.
Database link: P61160
- IP: HEK-293T whole cell lysate. IHC-P: Human skin squamous cell carcinoma.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab226467 was affinity purified using an epitope specific to Arp2 immobilized on solid support.
Our Abpromise guarantee covers the use of ab226467 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at 2-10 µg/mg of lysate.|
|IHC-P||1/1000 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionFunctions as ATP-binding component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks. Seems to contact the pointed end of the daughter actin filament.
Sequence similaritiesBelongs to the actin family. ARP2 subfamily.
Cellular localizationCytoplasm > cytoskeleton. Cell projection.
- Information by UniProt
- Actin like protein 2 antibody
- Actin related protein 2 antibody
- Actin-like protein 2 antibody
Arp2 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab226467 at 6 µg per reaction. Western blot was performed from the immunoprecipitate using ab226467 at 1 µg/ml.
Lane 1: ab226467 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG IP in HEK-293T whole cell lysate.
Detection: Chemiluminescence with exposure time of 10 seconds.
Cells prepared using NETN lysis buffer.
Formalin-fixed, paraffin-embedded human skin squamous cell carcinoma tissue stained for Arp2 using ab226467 at 1/5000 dilution in immunohistochemical analysis.
Detection: DAB staining. Counterstain: Hematoxylin (blue).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab226467 has not yet been referenced specifically in any publications.