Overview

  • Product name

  • Description

    Rabbit polyclonal to Arp2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Chicken, Cow, Xenopus laevis, Zebrafish, Orangutan, Xenopus tropicalis
  • Immunogen

    Synthetic peptide within Human Arp2 aa 25-75. The exact sequence is proprietary.
    Database link: P61160

  • Positive control

    • WB: HeLa, HEK-293T, Jurkat and NIH/3T3 cell lysate. IHC: Human skin squamous carcinoma tissue. IP: HEK-293T cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab226476 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 45 kDa.
IP Use at 2-10 µg/mg of lysate.
IHC-P 1/1000 - 1/5000.

Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.

Target

Images

  • All lanes : Anti-Arp2 antibody (ab226476) at 0.1 µg/ml

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
    Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
    Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate
    Lane 4 : TCMK-1 (mouse kidney epithelial cell line) cell lysate
    Lane 5 : NIH/3T3 (mouse embryo fibroblast cell line) cell lysate

    Lysates/proteins at 50 µg per lane.

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 45 kDa


    Exposure time: 10 seconds
  • Arp2 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (prepared using NETN buffer; 1.0 mg for IP, 20% of IP loaded) with ab226476 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab226476 at 1 µg/ml.

    Lane 1: ab226476 IP in HEK-293T whole cell lysate.

    Lane 2: Control IgG IP in HEK-293T whole cell lysate.

    Detection:  Chemiluminescence with exposure time of 10 seconds.

  • Human skin squamous carcinoma tissue stained for Arp2 using ab226476 at 1/5000 dilution in immunohistochemical analysis.

References

ab226476 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab226476.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up