Key features and details
- Rabbit polyclonal to Arp2
- Suitable for: WB, IP, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-Arp2 antibody
See all Arp2 primary antibodies
DescriptionRabbit polyclonal to Arp2
Tested applicationsSuitable for: WB, IP, IHC-Pmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Chicken, Cow, Xenopus laevis, Zebrafish, Orangutan, Xenopus tropicalis
Synthetic peptide within Human Arp2 aa 25-75. The exact sequence is proprietary.
Database link: P61160
- WB: HeLa, HEK-293T, Jurkat and NIH/3T3 cell lysate. IHC: Human skin squamous carcinoma tissue. IP: HEK-293T cell lysate.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab226476 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 45 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
|IHC-P||1/1000 - 1/5000.
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
FunctionFunctions as ATP-binding component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks. Seems to contact the pointed end of the daughter actin filament.
Sequence similaritiesBelongs to the actin family. ARP2 subfamily.
Cellular localizationCytoplasm > cytoskeleton. Cell projection.
- Information by UniProt
- Actin like protein 2 antibody
- Actin related protein 2 antibody
- Actin-like protein 2 antibody
All lanes : Anti-Arp2 antibody (ab226476) at 0.1 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate
Lane 4 : TCMK-1 (mouse kidney epithelial cell line) cell lysate
Lane 5 : NIH/3T3 (mouse embryo fibroblast cell line) cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 45 kDa
Exposure time: 10 seconds
Arp2 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (prepared using NETN buffer; 1.0 mg for IP, 20% of IP loaded) with ab226476 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab226476 at 1 µg/ml.
Lane 1: ab226476 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG IP in HEK-293T whole cell lysate.
Detection: Chemiluminescence with exposure time of 10 seconds.
Human skin squamous carcinoma tissue stained for Arp2 using ab226476 at 1/5000 dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab226476 has not yet been referenced specifically in any publications.