Overview

  • Product name

    Anti-Arp2 antibody [EPR7979]
    See all Arp2 primary antibodies
  • Description

    Rabbit monoclonal [EPR7979] to Arp2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, Flow Cytmore details
    Unsuitable for: ICC/IF,IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Arp2 aa 1-100. The exact sequence is proprietary.

  • Positive control

    • Jurkat, HUVEC, MCF-7, HeLa lysates. MCF-7 cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab129018 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 37 kDa (predicted molecular weight: 45 kDa).
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for ICC/IF,IHC-P or IP.
  • Target

    • Function

      Functions as ATP-binding component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks. Seems to contact the pointed end of the daughter actin filament.
    • Sequence similarities

      Belongs to the actin family. ARP2 subfamily.
    • Cellular localization

      Cytoplasm > cytoskeleton. Cell projection.
    • Information by UniProt
    • Database links

    • Alternative names

      • Actin like protein 2 antibody
      • Actin related protein 2 antibody
      • Actin-like protein 2 antibody
      • Actin-related protein 2 antibody
      • ACTR2 antibody
      • ARP2 actin related protein 2 homolog (yeast) antibody
      • ARP2 antibody
      • ARP2/3 COMPLEX antibody
      • ARP2_HUMAN antibody
      • OTTHUMP00000159937 antibody
      • OTTHUMP00000202270 antibody
      see all

    Images

    • All lanes : Anti-Arp2 antibody [EPR7979] (ab129018) at 1/1000 dilution

      Lane 1 : Jurkat cell lysates
      Lane 2 : HUVEC cell lysates
      Lane 3 : MCF-7 cell lysates
      Lane 4 : HeLa cell lysates

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 45 kDa
      Observed band size: 37 kDa
      why is the actual band size different from the predicted?

    • Flow cytometric analysis of permeabilized MCF-7 cells using ab129018 at 1/10 (red) or a rabbit IgG (negative) (green)
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    This product has been referenced in:

    • Mehedi M  et al. Actin-Related Protein 2 (ARP2) and Virus-Induced Filopodia Facilitate Human Respiratory Syncytial Virus Spread. PLoS Pathog 12:e1006062 (2016). Read more (PubMed: 27926942) »
    See 1 Publication for this product

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab129018.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Sign up