Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7979] to Arp2 - BSA and Azide free
- Suitable for: Flow Cyt, WB
- Reacts with: Mouse, Rat, Human
Product nameAnti-Arp2 antibody [EPR7979] - BSA and Azide free
See all Arp2 primary antibodies
DescriptionRabbit monoclonal [EPR7979] to Arp2 - BSA and Azide free
Tested applicationsSuitable for: Flow Cyt, WBmore details
Unsuitable for: ICC/IF,IHC-P or IP
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human Arp2 aa 1-100. The exact sequence is proprietary.
Ab248263 is the carrier-free version of ab129018. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
ab248263 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C. Do Not Freeze.
Dissociation constant (KD)KD = 1.75 x 10 -11 M Learn more about KD
Storage bufferConstituent: PBS
Concentration information loading...
Our Abpromise guarantee covers the use of ab248263 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use at an assay dependent concentration.|
|WB||Use at an assay dependent concentration. Detects a band of approximately 37 kDa (predicted molecular weight: 45 kDa).|
FunctionFunctions as ATP-binding component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks. Seems to contact the pointed end of the daughter actin filament.
Sequence similaritiesBelongs to the actin family. ARP2 subfamily.
Cellular localizationCytoplasm > cytoskeleton. Cell projection.
- Information by UniProt
- Actin like protein 2 antibody
- Actin related protein 2 antibody
- Actin-like protein 2 antibody
All lanes : Anti-Arp2 antibody [EPR7979] (ab129018) at 1/1000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : HUVEC cell lysate
Lane 3 : MCF-7 cell lysate
Lane 4 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 45 kDa
Observed band size: 37 kDa why is the actual band size different from the predicted?
This data was developed using ab129018, the same antibody clone in a different buffer formulation.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab248263 has not yet been referenced specifically in any publications.