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Signal Transduction Cytoskeleton / ECM Cytoskeleton Microfilaments Actin etc Actin Crosslinking
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RecombinantRabMAb

Recombinant Anti-Arp2 antibody [EPR7980] (ab128934)

  • Datasheet
  • SDS
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Western blot - Anti-Arp2 antibody [EPR7980] (ab128934)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Arp2 antibody [EPR7980] (ab128934)
  • OI-RD Scanning - Anti-Arp2 antibody [EPR7980] (ab128934)
  • Anti-Arp2 antibody [EPR7980] (ab128934)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR7980] to Arp2
  • Suitable for: WB, IHC-P
  • Reacts with: Human

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Overview

  • Product name

    Anti-Arp2 antibody [EPR7980]
    See all Arp2 primary antibodies
  • Description

    Rabbit monoclonal [EPR7980] to Arp2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide corresponding to residues in Human Arp2 (P61160).

  • Positive control

    • Human uterus tissue; Jurkat, Huvec and MCF7 cell lysates.
  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Dissociation constant (KD)

    KD = 7.00 x 10 -11 M
    Learn more about KD
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR7980
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microfilaments
    • Actin etc
    • Actin Crosslinking
    • Cancer
    • Invasion/microenvironment
    • ECM
    • Cell adhesion
    • Other

Associated products

  • Alternative Versions

    • Anti-Arp2 antibody [EPR7980] - BSA and Azide free (ab240037)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • Jurkat cytoplasmic extract lysate (ab14842)
    • Jurkat whole cell lysate (ab7899)
  • Recombinant Protein

    • Recombinant Human Arp2 protein (Tagged) (ab217837)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab128934 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/10000. Detects a band of approximately 37 kDa (predicted molecular weight: 45 kDa).
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Notes
WB
1/1000 - 1/10000. Detects a band of approximately 37 kDa (predicted molecular weight: 45 kDa).
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt or IP.
  • Target

    • Function

      Functions as ATP-binding component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks. Seems to contact the pointed end of the daughter actin filament.
    • Sequence similarities

      Belongs to the actin family. ARP2 subfamily.
    • Cellular localization

      Cytoplasm > cytoskeleton. Cell projection.
    • Target information above from: UniProt accession P61160 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 10097 Human
      • Entrez Gene: 66713 Mouse
      • Entrez Gene: 289820 Rat
      • Omim: 604221 Human
      • SwissProt: P61160 Human
      • SwissProt: P61161 Mouse
      • SwissProt: Q5M7U6 Rat
      • Unigene: 643727 Human
      • Unigene: 259045 Mouse
      • Unigene: 102249 Rat
      see all
    • Alternative names

      • Actin like protein 2 antibody
      • Actin related protein 2 antibody
      • Actin-like protein 2 antibody
      • Actin-related protein 2 antibody
      • ACTR2 antibody
      • ARP2 actin related protein 2 homolog (yeast) antibody
      • ARP2 antibody
      • ARP2/3 COMPLEX antibody
      • ARP2_HUMAN antibody
      • OTTHUMP00000159937 antibody
      • OTTHUMP00000202270 antibody
      see all

    Images

    • Western blot - Anti-Arp2 antibody [EPR7980] (ab128934)
      Western blot - Anti-Arp2 antibody [EPR7980] (ab128934)
      All lanes : Anti-Arp2 antibody [EPR7980] (ab128934) at 1/1000 dilution

      Lane 1 : Jurkat cell lysate
      Lane 2 : HUVEC cell lysate
      Lane 3 : MCF7 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Standard HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 45 kDa
      Observed band size: 37 kDa why is the actual band size different from the predicted?

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Arp2 antibody [EPR7980] (ab128934)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Arp2 antibody [EPR7980] (ab128934)

      ab128934, at a 1/100 dilution, staining Arp2 in paraffin-embedded human uterus tissue by immunohistochemistry.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • OI-RD Scanning - Anti-Arp2 antibody [EPR7980] (ab128934)
      OI-RD Scanning - Anti-Arp2 antibody [EPR7980] (ab128934)
      Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD
    • Anti-Arp2 antibody [EPR7980] (ab128934)
      Anti-Arp2 antibody [EPR7980] (ab128934)

    Protocols

    • Immunohistochemistry protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (5)

    Publishing research using ab128934? Please let us know so that we can cite the reference in this datasheet.

    ab128934 has been referenced in 5 publications.

    • Bolger-Munro M  et al. Arp2/3 complex-driven spatial patterning of the BCR enhances immune synapse formation, BCR signaling and B cell activation. Elife 8:N/A (2019). PubMed: 31157616
    • Miller HE  et al. Actin polymerization in the endosomal pathway, but not on the Coxiella-containing vacuole, is essential for pathogen growth. PLoS Pathog 14:e1007005 (2018). PubMed: 29668757
    • Braun DA  et al. Mutations in KEOPS-complex genes cause nephrotic syndrome with primary microcephaly. Nat Genet 49:1529-1538 (2017). PubMed: 28805828
    • Kahr WH  et al. Loss of the Arp2/3 complex component ARPC1B causes platelet abnormalities and predisposes to inflammatory disease. Nat Commun 8:14816 (2017). PubMed: 28368018
    • He SW  et al. SKAP2 regulates Arp2/3 complex for actin-mediated asymmetric cytokinesis by interacting with WAVE2 in mouse oocytes. Cell Cycle 16:2272-2281 (2017). PubMed: 28933599

    Customer reviews and Q&As

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