Recombinant
RabMAb

Recombinant Anti-Arp2 antibody [EPR7980] (ab128934)

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Overview

  • Product name

    Anti-Arp2 antibody [EPR7980]
    See all Arp2 primary antibodies

  • Description

    Rabbit monoclonal [EPR7980] to Arp2

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
    Unsuitable for: Flow Cyt or IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide corresponding to residues in Human Arp2 (P61160).

  • Positive control

    • Human uterus tissue; Jurkat, Huvec and MCF7 cell lysates.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

Our Abpromise guarantee covers the use of ab128934 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 37 kDa (predicted molecular weight: 45 kDa).
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF 1/250 - 1/500.
  • Application notes
    Is unsuitable for Flow Cyt or IP.

    • Target

    • Function

      Functions as ATP-binding component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks. Seems to contact the pointed end of the daughter actin filament.

    • Sequence similarities

      Belongs to the actin family. ARP2 subfamily.

    • Cellular localization

      Cytoplasm > cytoskeleton. Cell projection.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • Actin like protein 2 antibody
      • Actin related protein 2 antibody
      • Actin-like protein 2 antibody
      • Actin-related protein 2 antibody
      • ACTR2 antibody
      • ARP2 actin related protein 2 homolog (yeast) antibody
      • ARP2 antibody
      • ARP2/3 COMPLEX antibody
      • ARP2_HUMAN antibody
      • OTTHUMP00000159937 antibody
      • OTTHUMP00000202270 antibody
      see all

    Images

    • Western blot - Anti-Arp2 antibody [EPR7980] (ab128934)
      Western blot - Anti-Arp2 antibody [EPR7980] (ab128934)
      All lanes : Anti-Arp2 antibody [EPR7980] (ab128934) at 1/1000 dilution

      Lane 1 : Jurkat cell lysate
      Lane 2 : HUVEC cell lysate
      Lane 3 : MCF7 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Standard HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 45 kDa
      Observed band size: 37 kDa
      why is the actual band size different from the predicted?

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Arp2 antibody [EPR7980] (ab128934)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Arp2 antibody [EPR7980] (ab128934)

      ab128934, at a 1/100 dilution, staining Arp2 in paraffin-embedded human uterus tissue by immunohistochemistry.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Other - Anti-Arp2 antibody [EPR7980] (ab128934)
      Other - Anti-Arp2 antibody [EPR7980] (ab128934)
      Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    This product has been referenced in:

    • Bolger-Munro M  et al. Arp2/3 complex-driven spatial patterning of the BCR enhances immune synapse formation, BCR signaling and B cell activation. Elife 8:N/A (2019). Read more (PubMed: 31157616) »
    • Miller HE  et al. Actin polymerization in the endosomal pathway, but not on the Coxiella-containing vacuole, is essential for pathogen growth. PLoS Pathog 14:e1007005 (2018). Read more (PubMed: 29668757) »
    See all 5 Publications for this product

    Publishing research using ab128934? Please let us know so that we can cite the reference in this datasheet.

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