Recombinant Anti-Arp2 antibody [EPR7980] (ab128934)
![Western blot - Anti-Arp2 antibody [EPR7980] (ab128934)](https://www.abcam.com/ps/products/128/ab128934/Images/ab128934-142794-anti-arp2-antibody-epr7980-western-blot.jpg "Western blot - Anti-Arp2 antibody [EPR7980] (ab128934)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7980] to Arp2
- Suitable for: WB, IHC-P
- Reacts with: Human
Overview
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Product name
Anti-Arp2 antibody [EPR7980]
See all Arp2 primary antibodies -
Description
Rabbit monoclonal [EPR7980] to Arp2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt or IP -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Synthetic peptide corresponding to residues in Human Arp2 (P61160).
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Positive control
- Human uterus tissue; Jurkat, Huvec and MCF7 cell lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 7.00 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR7980 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab128934 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000 - 1/10000. Detects a band of approximately 37 kDa (predicted molecular weight: 45 kDa).
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| IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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| Notes |
|---|
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WB
1/1000 - 1/10000. Detects a band of approximately 37 kDa (predicted molecular weight: 45 kDa). |
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IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Application notes
Is unsuitable for Flow Cyt or IP.
Target
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Function
Functions as ATP-binding component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks. Seems to contact the pointed end of the daughter actin filament. -
Sequence similarities
Belongs to the actin family. ARP2 subfamily. -
Cellular localization
Cytoplasm > cytoskeleton. Cell projection. - Information by UniProt
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Database links
- Entrez Gene: 10097 Human
- Entrez Gene: 66713 Mouse
- Entrez Gene: 289820 Rat
- Omim: 604221 Human
- SwissProt: P61160 Human
- SwissProt: P61161 Mouse
- SwissProt: Q5M7U6 Rat
- Unigene: 643727 Human
see all -
Alternative names
- Actin like protein 2 antibody
- Actin related protein 2 antibody
- Actin-like protein 2 antibody
see all
Images
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Western blot - Anti-Arp2 antibody [EPR7980] (ab128934)All lanes : Anti-Arp2 antibody [EPR7980] (ab128934) at 1/1000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : HUVEC cell lysate
Lane 3 : MCF7 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Standard HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 45 kDa
Observed band size: 37 kDa why is the actual band size different from the predicted? -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Arp2 antibody [EPR7980] (ab128934)](https://www.abcam.com/ps/products/128/ab128934/Images/ab128934-142793-anti-arp2-antibody-epr7980-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Arp2 antibody [EPR7980] (ab128934)ab128934, at a 1/100 dilution, staining Arp2 in paraffin-embedded human uterus tissue by immunohistochemistry.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![OI-RD Scanning - Anti-Arp2 antibody [EPR7980] (ab128934)](https://www.abcam.com/ps/products/128/ab128934/Images/ab128934-142792-anti-arp2-antibody-epr7980.jpg)
OI-RD Scanning - Anti-Arp2 antibody [EPR7980] (ab128934) -
![Anti-Arp2 antibody [EPR7980] (ab128934)](https://www.abcam.com/ps/products/128/ab128934/Images/ab128934-4-benefits-of-recombinant-antibodies.png)
Anti-Arp2 antibody [EPR7980] (ab128934)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (5)
ab128934 has been referenced in 5 publications.
- Bolger-Munro M et al. Arp2/3 complex-driven spatial patterning of the BCR enhances immune synapse formation, BCR signaling and B cell activation. Elife 8:N/A (2019). PubMed: 31157616
- Miller HE et al. Actin polymerization in the endosomal pathway, but not on the Coxiella-containing vacuole, is essential for pathogen growth. PLoS Pathog 14:e1007005 (2018). PubMed: 29668757
- Braun DA et al. Mutations in KEOPS-complex genes cause nephrotic syndrome with primary microcephaly. Nat Genet 49:1529-1538 (2017). PubMed: 28805828
- Kahr WH et al. Loss of the Arp2/3 complex component ARPC1B causes platelet abnormalities and predisposes to inflammatory disease. Nat Commun 8:14816 (2017). PubMed: 28368018
- He SW et al. SKAP2 regulates Arp2/3 complex for actin-mediated asymmetric cytokinesis by interacting with WAVE2 in mouse oocytes. Cell Cycle 16:2272-2281 (2017). PubMed: 28933599