Overview

  • Product name

    Anti-Arp3 antibody [EPR10428(B)]
    See all Arp3 primary antibodies
  • Description

    Rabbit monoclonal [EPR10428(B)] to Arp3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC/IF or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Arp3 aa 50-150. The exact sequence is proprietary.

  • Positive control

    • Jurkat, HeLa, A431 and A549 cell lysates; Human tonsil and Mouse brain tissues.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab151729 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 47 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IP.
  • Target

    • Relevance

      The Arp2/3 complex involved in Golgi polerization in NIH3T3 cells. In a different model it has been shown that the Arp2/3 complex is necessary for neutrophil chemotaxis and phagocytosis.
    • Cellular localization

      Cytoplasmic
    • Database links

    • Alternative names

      • Actin like protein 3 antibody
      • Actin related protein 3 antibody
      • ACTR3 antibody
      • ARP3 actin related protein 3 homolog (yeast) antibody

    Images

    • All lanes : Anti-Arp3 antibody [EPR10428(B)] (ab151729) at 1/1000 dilution

      Lane 1 : Jurkat cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : A431 cell lysate
      Lane 4 : A549 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 47 kDa

    • Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling Arp3 with ab151729 at 1/50 dilution.
    • Immunohistochemical analysis of paraffin-embedded Mouse brain tissue labeling Arp3 with ab151729 at 1/50 dilution.

    References

    This product has been referenced in:

    • Schell C  et al. ARP3 Controls the Podocyte Architecture at the Kidney Filtration Barrier. Dev Cell 47:741-757.e8 (2018). Read more (PubMed: 30503751) »
    • Kahr WH  et al. Loss of the Arp2/3 complex component ARPC1B causes platelet abnormalities and predisposes to inflammatory disease. Nat Commun 8:14816 (2017). Read more (PubMed: 28368018) »
    See all 2 Publications for this product

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab151729.
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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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