Key features and details
- Rabbit polyclonal to ARPC1A
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-ARPC1A antibody
See all ARPC1A primary antibodies
DescriptionRabbit polyclonal to ARPC1A
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Chicken, Cow, Pig
Recombinant fragment within Human ARPC1A (internal sequence). The exact sequence is proprietary.
Database link: Q92747
- IHC-P: Human lung carcinoma tissue. WB: ARPC1A transfected HEK-293T whole cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab227931 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/5000 - 1/20000. Predicted molecular weight: 42 kDa.|
|IHC-P||1/100 - 1/1000.|
FunctionProbably functions as component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks.
Sequence similaritiesBelongs to the WD repeat ARPC1 family.
Contains 6 WD repeats.
Cellular localizationCytoplasm > cytoskeleton.
- Information by UniProt
- Actin binding protein (Schizosaccharomyces pombe sop2 like) antibody
- Actin related protein 2/3 complex subunit 1A 41kDa antibody
- Actin-related protein 2/3 complex subunit 1A antibody
Paraffin-embedded human lung carcinoma tissue stained for ARPC1A using ab227931 at 1/500 dilution in immunohistochemical analysis.
All lanes : Anti-ARPC1A antibody (ab227931) at 1/10000 dilution
Lane 1 : Non-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : ARPC1A transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 42 kDa
10% SDS-PAGE gel.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab227931 has not yet been referenced specifically in any publications.