Key features and details
- Rabbit polyclonal to ARPC1B
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-ARPC1B antibody
See all ARPC1B primary antibodies
DescriptionRabbit polyclonal to ARPC1B
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cow, Pig
Recombinant fragment within Human ARPC1B (internal sequence). The exact sequence is proprietary.
Database link: O15143
- WB: A431 whole cell lysate. ICC/IF: A431 cells.
This product was previously labelled as ARP2/3 subunit 1B
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab227427 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 41 kDa.|
|ICC/IF||1/100 - 1/1000.|
FunctionFunctions as component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks.
Sequence similaritiesBelongs to the WD repeat ARPC1 family.
Contains 6 WD repeats.
Cellular localizationCytoplasm > cytoskeleton.
- Information by UniProt
- Actin related protein 2/3 complex subunit 1B 41kDa antibody
- Actin related protein 2/3 complex subunit 1B antibody
- Actin-related protein 2/3 complex subunit 1B antibody
Methanol-fixed A431 (human epidermoid carcinoma cell line) cells stained for ARPC1B using ab227427 (green) at 1/500 dilution in ICC/IF. Counterstained with Hoechst 33342 (blue).
Anti-ARPC1B antibody (ab227427) at 1/500 dilution + A431 (human epidermoid carcinoma cell line) whole cell lysate at 30 µg
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 41 kDa
10% SDS PAGE
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab227427 has not yet been referenced specifically in any publications.